Introduction . Blood transfusion is a strong practice in traumatology, internal medicine, haematology, obstetrics and transplantation, which demands safety of haemotransfusion with estimating the red blood cell group antigens donor recipient blood. Routine immunotyping techniques usually provide for an antigen identification to weak subgroups, albeit certain inherent limitations serology tests ...

In the last decade, the revolution in sequencing technologies has deeply impacted crop genotyping practice. New methods allowing rapid, high-throughput genotyping of entire crop populations have proliferated and opened the door to wider use of molecular tools in plant breeding. These new genotyping-by-sequencing (GBS) methods include over a dozen reduced-representation sequencing (RRS) approach...

Conventional genotyping-by-sequencing (cGBS) strategies suffer from high rates of missing data and genotyping errors, particularly at heterozygous sites. tGBS® genotyping-by-sequencing is a novel method of genome reduction that employs two restriction enzymes to generate overhangs in opposite orientations to which (single-strand) oligos rather than (double-stranded) adaptors are ligated. This s...

Microbial genotyping increasingly deals with large numbers of samples, and data are commonly evaluated by unstructured approaches, such as spread-sheets. The efficiency, reliability and throughput of genotyping would benefit from the automation of manual manipulations within the context of sophisticated data storage. We developed a medium- throughput genotyping pipeline for MultiLocus Sequence ...

background: avian infectious bronchitis (ib), with avianinfectious bronchitis virus (ibv) as the causing agent, is a ubiquitous endemicdisease of the chicken with devastating effects on its industry. a viralmembrane surface protein called s  notonly induces neutralizing antibodies but also plays an important role in virusbinding and entry to host cells. technically, s1 protein gene sequencing a...

conclusions findings of the present study showed that hcv genotype 3 was the predominant genotype followed by the subtypes 1a and 1b in yazd, central province of iran. in addition, there was no difference between hcv load and genotypes 1 and 3. hcv genotyping is recommended in other provinces of iran. objectives the main aim of this study was to estimate the prevalence of hcv genotypes in yazd,...

conclusions cervical cancer screening, comprising of both pap test and hpv testing, is an essential part of the routine health care for women as it can facilitate the detection of abnormal cervical cells. hpv infection can produce abnormal pap test results; also, certain types of hpv are associated with cancer. therefore, it is important to diagnose abnormal cervical tissues before the developm...

conclusions: our findings suggest the possible co-carriage of some blashv genes and aris on the same plasmids harboring the mdr genes. possible role of integrons in dissemination of esbl-encoding blashv genes among esbl-producing k. pneumoniae nosocomial isolates may be inferred. background: some genetic and phenotypic variables are associated among distinct microbial populations. objectives: t...

diacylglycerol acyltransferase1 (dgat1) has been introduced as a strong potential candidate gene for milk fat content in cattle. dgat1 gene plays a key role in the final step of triglyceride synthesis. in this study, the genetic variability in the region spanning exon 1 to exon 2 of the buffalo dgat1 gene was investigated by dna sequencing. two hundred buffaloes belonging to three indigenous po...

Objectives: Accurately identifying the Antigens (Ags) on recipient red blood cells (RBCs) is critical in prevention of RBC alloimmunization in chronically transfused patients. The goal of this study was to compare RBC molecular genotyping to serological phenotyping in those patients. Methods: Serological phenotyping and molecular genotyping methods were used to study blood samples from 18 healt...