Reliability of genotyping is an issue for studies using non-invasive sources of DNA. We emphasize the importance of refining DNA extraction methods to maximize reliability and efficiency of genotyping for such DNA sources. We present a simple and general method to quantitatively compare genotyping reliability of various DNA extraction techniques and sample materials used. For bighorn sheep (Ovi...

Abstract Each year, 75–100 unprovoked shark attacks on humans are recorded, most of them resulting in no or minor injuries, while a few fatal. Often, identification responsible for relies visual observations bite wound characteristics, which limits species determination and preclude individual identification. Here, we provide two genetic approaches to reliably identify and/or individua...

Current techniques for single nucleotide polymorphism (SNP) detection require tedious experimental procedures and expensive and sophisticated instruments. In this study, a visual genotyping method has been successfully established via combining ARMS-PCR with gold magnetic nanoparticle (GoldMag)-based lateral flow assay (LFA) and applied to the genotyping of methylenetetrahydrofolate reductase (...

In 2000, we monitored the course and persistence of human papillomavirus (HPV) infection in 54 women who were HPV positive and free of any cytological disease using HPV-DNA genotyping with a linear array assay (baseline). The impact of HPV infection on development of cervical cytological abnormality (dyskaryosis) was monitored by repeat HPV genotyping and cytological assessment 2 years later. D...

Noninvasive faecal DNA sampling has the potential to provide a wealth of information necessary for monitoring and managing endangered species while eliminating the need to capture, handle or observe rare individuals. However, scoring problems, and subsequent genotyping errors, associated with this monitoring method remain a great concern as they can lead to misidentification of individuals and ...

background: hepatitis c is a disease with significant global impact. the distribution of hepatitis c virus (hcv) genotypes in mashhad (the northeast and the biggest city after the capital of iran) is unknown. the purpose of this study was to determine the prevalence of hcv genotypes among hcv seropositive patients, and to study the relationship between types, virologic and demographic features ...