T HE ENZYME glutathione peroxidase appears to play a major role in the red cell in the detoxification of potential oxidizing agents. A genetically determined partial, or presumably heterozygous, deficiency of this enzyme has been associated with a transient hemolytic anemia and hyperbilirubinemia in newborns.l* Unless subjected to oxidative stress, red cells from older children and adults with ...

Peroxiredoxin VI (PrxVI) is a bifunctional enzyme with non-selenium glutathione peroxidase and Ca2+-independent acidic phospholipase A2 activities. We demonstrate that transfection-mediated PrxVI overexpression protects immortalized human WI-38 and murine NIH3T3 fibroblasts against cytotoxic doses of tert-butylhydroperoxide and H2O2. Mutants for either glutathione peroxidase or phospholipase A2...

We compared four methods for determination of glutathione peroxidase (EC 1.11.1.9) activity, using blood samples from 52 healthy volunteers. Two methods depended on direct assay of the amount of glutathione remaining at specific time intervals; the two indirect methods involved measuring the rate of disappearance of NADPH. We assessed the precision and reproducibility of each method. One of the...

T HE ENZYME glutathione peroxidase appears to play a major role in the red cell in the detoxification of potential oxidizing agents. A genetically determined partial, or presumably heterozygous, deficiency of this enzyme has been associated with a transient hemolytic anemia and hyperbilirubinemia in newborns.l* Unless subjected to oxidative stress, red cells from older children and adults with ...

The major aim of this work was to investigate how alcohol-induced oxidative stress in combined chemotherapy changes the metabolic function of the liver in experimental animals. This research was conducted to establish how bromocriptine, haloperidol and azithromycin, applied to the experimental model, affected the antioxidative status of the liver. The following parameters were determined: reduc...