background and objective: microbial protein production can resolve one of the major world challenges, i.e. lack of protein sources. candida tropicalis growth was investigated to specify a medium to reach the highest cell proliferation and protein production. material and methods: fractional factorial design and the index of signal to noise ratio were applied for optimization of microbial protei...

These experiments were designed to investigate the defect in amino acid uptake in a D-serine-resistant mutant (WS) of Escherichia coli W that is deficient in the uptake of glycine. Under conditions in which there is insignificant metabolism of glycine (i.e. 0” under nitrogen), the wild type took up significant amounts of glycine, whereas the WS mutant did not equilibrate with the external glyci...

I . Rates of absorption of glycine and glycylglycine from a 300 mm jejunal segment were compared in vivo when those compounds were given alone or together to six Zambian African subjects who had no clinical evidence of malnutrition or of gastro-intestinal disease. Solutions containing (A) glycine (~oommol/l), (B) glycine (Ioommol/l)+glycylglycine (50 mmol/l), and (C) glycglglycinc (50 mmolil) w...

BACKGROUND Human fetal midbrain-derived neural progenitor cells (NPCs) may deliver a tissue source for drug screening and regenerative cell therapy to treat Parkinson's disease. While glutamate and GABA(A) receptors play an important role in neurogenesis, the involvement of glycine receptors during human neurogenesis and dopaminergic differentiation as well as their molecular and functional cha...

Glycine decarboxylase, tentatively called P-protein and considered a constituent of the glycine cleavage system, was purified to apparent homogeneity from chicken liver mitochondria. P-protein is a homodimer having a Mr = approximately 200,000 and consisting of identical subunits with Mr = approximately 100,000. Each subunit appears to contain an equimolar pyridoxal 5'-phosphate which is bound ...

Glycine, an important inhibitory neurotransmitter in the mammalian central nervous system (CNS), has been shown to modulate peripheral immune cell responses. In that respect, glycine levels are increased in several neuroinflammatory disorders, such as amyotrophic lateral sclerosis (ALS) and multiple sclerosis (MS). In this study, we show that glycine modulates macrophage effector functions impl...

These experiments were designed to investigate the defect in amino acid uptake in a D-serine-resistant mutant (WS) of Escherichia coli W that is deficient in the uptake of glycine. Under conditions in which there is insignificant metabolism of glycine (i.e. 0” under nitrogen), the wild type took up significant amounts of glycine, whereas the WS mutant did not equilibrate with the external glyci...

The anticonvulsant compound felbamate (2-phenyl-1,3-propanediol dicarbamate; FBM) appears to inhibit the function of the N-methyl-D-aspartate (NMDA) receptor complex through an interaction with the strychnine-insensitive glycine recognition site. Since we have demonstrated previously that FBM inhibits the binding of [3H]5, 7-dichlorokynurenic acid (DCKA), a competitive antagonist at the glycine...

Christensen, Riggs, and their coworkers (l-5) showed that the Ehrlich mouse ascites tumor cell concentrated amino acids to values many times that of the environment. Glycine, for example, was concentrated to 16 times that of the external solution. Heinz (6-8) studied the kinetics of glycine accumulation and showed that active transport across the cell membrane was responsible for the concentrat...