Neonatal bacterial infection is diagnosed when the acute phase reactants score (APR-Sc) positive for 3 items of CRP, α1-acid glycoprotein (AGP), and haptoglobin (Hp), 2 points are items, CRP AGP. However, mechanism by which presence or absence an increase in Hp, difference between points, not known.

The urinary bladder is lined by transitional epithelium, the glycocalyx on the luminal surface has interesting properties and is implicated in protective functions. Glycoconjugates are major components of the glycocalyx, but their biochemical nature is not well understood. Previous studies on rabbit bladder indicated the presence of significant levels of sialoglycoproteins compared to glycosami...

Glycan heterogeneity and subunit composition of therapeutic glycoprotein complexes are challenging to define. In their Communication (e202213170), Di Wu Carol V. Robinson present a native top-down mass spectrometry approach, together with in silico modelling, reveal the stabilization effect interfacial N-glycans on cytokine hormone assemblies.

The glycoproteins of human erythrocyte membranes were crosslinked with dimethyl adipimidate dihydrochloride. On sodium dodecyl sulfate-polyacrylamide gel electrophoregrams of the crosslinked solubilized membranes, at least three new glycoprotein complexes appeared in addition to the normal glycoprotein species. One of the new glycoprotein complexes was shown to contain two of the three species ...

Although, generally speaking, haematological malignancies are chemotherapy-responsive tumours and high remission induction rates are obtained, disease-related death is the rule rather than the exception. The appearance of cell populations, resistant to multidrug-based chemotherapy, constitutes the major problem to achieve cures in these patients. Advances in cell biology have partly contributed...

The M, 170,000 to 180,000 membrane glycoprotein associated with multidrug resistance (P-glycoprotein) is involved in drug transport mech anisms across the plasma membrane of multidrug-resistant cells. We have recently reported the purificai inn of P-glycoprotein. The purified Pglycoprotein was found to have an ATPase activity, which might be coupled with the active efflux of anticancer drugs. I...

Multidrug resistance (MDR) remains a major obstacle to successful chemotherapeutic treatment of cancer and can be caused by overexpression of P-glycoprotein, the MDR1 gene product. To further validate a knockdown approach for circumventing MDR, we developed a P-glycoprotein inhibition strategy using short hairpin RNA interference (shRNAi) and now show efficacy and target specificity in vivo. Tw...

Development of biosimilars is costly, where glycan analysis a significant constraint on time and money. This paper provides an in-depth characterisation several novel recombinant prokaryotic lectins (RPLs), developed through directed evolution, displaying specific binding activities to ?-mannose, ?-galactose, fucose sialic acid residues, tested against major biosimilar targets. The all was perf...

Human CD46 is a cellular receptor for human herpesvirus 6 (HHV-6). Virus entry into host cells requires a glycoprotein H (gH)-glycoprotein L (gL) complex. We show that the CD46 ectodomain blocked HHV-6 infection and bound a complex of gH-gL and the 80-kDa U100 gene product, designated glycoprotein Q, indicating that the complex is a viral ligand for CD46.

The aim of this study was to examine the peptide transport activity of a naturally occurring P-glycoprotein such as that present in rat liver canalicular membrane vesicles. The peptide ionophores valinomycin and gramicidin D, which are known substrates of P-glycoprotein, served to monitor the P-glycoprotein activity indirectly as the ATP-dependent uptake of 86Rb+ mediated by these ionophores. C...