Multidrug resistance protein 1 (MRP1/ABCC1) is an ATP-dependent polytopic membrane protein that transports many anticancer drugs and organic anions. Its transport mechanism is multifaceted, especially with respect to the participation of GSH. For example, vincristine is cotransported with GSH, estrone sulfate transport is stimulated by GSH, or MRP1 can transport GSH alone, and this can be stimu...

Dihalomethanes can produce liver tumors in mice but not in rats, and concern exists about the risk of these compounds to humans. Glutathione (GSH) conjugation of dihalomethanes has been considered to be a critical event in the bioactivation process, and risk assessment is based upon this premise; however, there is little experimental support for this view or information about the basis of genot...

We investigated the hypothesis that reduced glutathione (GSH) is present in secretions of the female reproductive tract and that this extracellular GSH may protect preimplantation mouse embryos after intracellular GSH depletion. The cleavage-stage mouse embryo cannot synthesize GSH de novo and is unable to recover from glutathione depletion in vitro. Analysis of GSH and total protein of oviduct...

This work is the next step in studying interplay between C-2028 (anticancer-active unsymmetrical bisacridine developed our group) and glutathione S-transferase/glutathione (GST/GSH) system. Here, we analyzed concentration- pH-dependent GSH conjugation of rat liver microsomes cytosol. We also applied three recombinant human GST isoenzymes, which altered expression was found various tumors. The f...

Glutathione (GSH) is a major source of reducing equivalents in mammalian cells. To examine the role of GSH synthesis in development and cell growth, we generated mice deficient in GSH by a targeted disruption of the heavy subunit of gamma-glutamylcysteine synthetase (gammaGCS-HS(tm1)), an essential enzyme in GSH synthesis. Embryos homozygous for gammaGCS-HS(tm1) fail to gastrulate, do not form ...

A cognitive-behavioral guided self-help conducted by lay providers (CBT-GSH-LP) had been shown to be effective in treating anxiety and may help facilitate access treatment. The first objective of this study was assess the acceptability CBT-GSH-LP for Generalised Anxiety Disorder adults aged 60 over. Its compared that same treatment a psychotherapist (CBT- GSH-PSY) face-to-face cognitive behavio...

PURPOSE To characterize glutathione (GSH) transport by cultured human retinal pigment epithelial (HRPE) cells. METHODS Cultured HRPE cells were pretreated with acivicin for GSH efflux and with buthionine sulfoximine for GSH uptake to prevent the breakdown and resynthesis of GSH. Efflux was measured by the linear rate of accumulation of GSH in the supernatant; uptake was measured using [35S] G...

tion from toxic oxygen species and detoxification of various xenobiotics (8, 9). The ability to modulate cellular GSH levels to probe the importance of this tripeptide in various drug or radiation interactions hascome from the elegant work of Meis ter and Griffith (10) and Williamson et a!. (11) who have introduced compounds that either inhibit or stimulate GSH synthesis. When GSH synthesis is ...

The cystic fibrosis transmembrane conductance regulator (CFTR) protein is the only known apical glutathione (GSH) transporter in the lung. The purpose of these studies was to determine whether oral GSH or glutathione disulfide (GSSG) treatment could increase lung epithelial lining fluid (ELF) GSH levels and whether CFTR plays a role in this process. The pharmacokinetic profile of an oral bolus ...