TFIIB (transcription factor IIB) is a transcription factor that provides a bridge between promoter-bound TFIID and RNA polymerase II, and it is a target of various transcriptional activator proteins that stimulate the pre-initiation complex assembly. The localization and/or attachment matrix of TFIIB in the cytoplast is not well understood. This study focuses on the function of TFIIB and its in...

Mitochondria (mt) have been reported to be closely related to the maturation of mammalian oocytes, but their function in oocyte maturation has not been elucidated. In this study, we examined the kinetics of mt and chromatin configuration during in vitro maturation of mouse oocytes to clarify the relationship between oocyte maturation and mitochondrial distribution morphologically. Oocytes were ...

objectives: it was aimed to investigate the effects of different doses of follicle stimulating hormone (fsh) and activin a on the growth and maturation of preantral mouse follicles during the in vitro culture. methods: preantral follicles (90-100 µm in diameter) were harvested from 6-8 week-old syrian mice and cultured in tcm199 culture medium for 6 days to see the effect of fsh and activin a. ...

Abstract Study question Whether follicular extracellular vesicles of older women interfere with the quality oocytes. Summary answer OLD-EVs induced oxidative stress in oocytes and inhibited oocyte maturation by increasing abnormal mitochondria distribution spindle rates. What is known already Ovary a critical regulator female fertility source With modern tendency delaying childbearing, ovarian ...

this study was inducted to elucidate the effect of different steroid hormones on simultaneous maturation of oocytes in caspian salmon. oocytes were incubated with 1, 10, 100 and 1000 ng/ml of testosterone, progesterone and 17 β stradiol, in vitro, for 40 hrs. gvbd was considered as hormones effectiveness. 17β estradiol was effective just in 1000 ng/ml and testosterone had no positive effect on ...

The relationship between the mos protooncogene protein and cAMP-dependent protein kinase (PKA) during the maturation of Xenopus oocytes was investigated. Microinjection of the PKA catalytic subunit (PKA0 into Xenopus oocytes inhibited oocyte maturation induced by the mos product but did not markedly affect the autophosphorylation activity of injected mos protein. By contrast, PKA~ did not inhib...

In the present study, we established an in vitro culture system suitable for generating fertilizable oocytes from premeiotic mouse female germ cells. These results were achieved after first establishing an in vitro culture system allowing immature oocytes from 12-14 day-old mice to reach meiotic maturation through culture onto preantral granulosa cell (PAGC) monolayers in the presence of Activi...