Since program approval, Niger has been facing a more challenging international context, including the fall-out from war in Ukraine, which is exacerbating pressures on food and fertilizer prices. Moreover, unfavorable rainfall deterioration security situation led to deceleration of growth 1.3 percent 2021 an acute crisis. A temporary deviation fiscal deficit targets 2022-23 needed respond urgent...

An a-galactosidase and a p-mannanase produced by the hyperthermophilic bacterium, Thermotoga neapolitana 5068 (TN50681, separately and together, were evaluated fortheir ability to hydrolyze guar gum in relation toviscosity reduction of guar-based hydraulic fracturing fluids used in oil and gas well stimulation. In such applications, premature guar gum hydrolysis at lower temperatures before the...

In order to improve the expression of heat-resistant xylanase XYNB from Aspergillus niger SCTCC 400264, XynB has been cloned into Pichia pastoris secretary vector pPIC9K. The XynB production of recombinant P. pastoris was four times as E. coli, the Vmax and specific activity of XynB reached 2,547.7 μmol/mg and 4,757 U/mg, respectively. And the XynB still had 74% residual enzyme activity after 3...

The present study deals with the pre-treatment of sugar cane molasses for the enhanced production of citric acid by Aspergillus niger NG-4. Different metal complexing agents such as potassium ferrocyanide [K4Fe(CN)6] and ethylene diamine tetra acetic acid (EDTA) were also employed for molasses pre-treatment and the concentrations varied from 50-350 ppm. The maximum production of citric acid (36...

In an effort to develop alternate techniques to recover metals from waste electrical and electronic equipment (WEEE), this research evaluated the bioleaching efficiency of gold (Au), copper (Cu) and nickel (Ni) by two strains of Aspergillus niger in the presence of gold-plated finger integrated circuits found in computer motherboards (GFICMs) and cellular phone printed circuit boards (PCBs). Th...

We observed that mouse spleen cells from rosettes with autologous red blood cells (RBCs) and that rosette-formation was suppressed by anti-Aspergillus niger glucose oxidase monoclonal antibody (mAb). In the present study, we investigated whether RBCs of species besides mice have the structure recognized by anti-A. niger glucose oxidase mAb by using rosette-formation and complement-mediated hemo...

The presence of a mannitol cycle in fungi has been subject to discussion for many years. Recent studies have found no evidence for the presence of this cycle and its putative role in regenerating NADPH. However, all enzymes of the cycle could be measured in cultures of Aspergillus niger. In this study we have analyzed the localization of two enzymes from the pathway, mannitol dehydrogenase and ...

On 4 August 2011, United Nations Environment Program (UNEP) submitted an unprecedented, scientific, groundbreaking environmental assessment report (EAR) on Ogoniland to the Nigerian government. This was the outcome of a 14-month intensive evaluation of the extent of pollution. The intention was that UNEP’s recommendations would be implemented to restore the devastated environment, on the one ha...

An in-depth exploration of the headspace content of Aspergillus niger cultures was performed upon different growth conditions, using a methodology based on advanced multidimensional gas chromatography. This volatile fraction comprises 428 putatively identified compounds distributed over several chemical families, being the major ones hydrocarbons, alcohols, esters, ketones and aldehydes. These ...

The asexual filamentous fungus Aspergillus niger is an important industrial cell factory for citric acid production. In this study, we genetically characterized a UV-generated A. niger mutant that was originally isolated as a nonacidifying mutant, which is a desirable trait for industrial enzyme production. Physiological analysis showed that this mutant did not secrete large amounts of citric a...