Objective: This study involved the formulation of protocol of Agrobacterium rhizogenes mediated transformation for the detailed study of isoflavones (IFs) metabolism in soybean. Methods: Cotyledons were separated from 4-day-old soybean seedlings and infected with three different A. rhizogenes strains under various time durations of sonication, vacuum infiltration and cocultivated on Murashige a...

An improved Agrobacterium rhizogenes-mediated genetic transformation of Withania somnifera (L.) Dunal was developed using the bacterial strain R1000 with leaf segment explants of in vitro raised plantlets. Out of the three strains used (R1000, MTCC 2364 and MTCC 532), the strain R1000 proved to be more efficient than others. Among the different conditions tested, the highest (93.3 %) transforma...

Feeding synthetic [2beta-2H]- and [2alpha-2H]-cholesterols to the hairy roots of Ajuga reptans var. atropurpurea and 2H-NMR analysis of the biosynthesized 20-hydroxyecdysone revealed that hydroxylation at C-2 proceeds with retention of configuration. Feeding [2alpha,3alpha-2H2]cholesterol followed by 2H-NMR analysis of the 2,3,22-triacetate of the resulting 20-hydroxyecdysone ruled out a mechan...

The Agrobacterium-mediated transformation of hairy roots creates a rapid and simple means to introduce and express foreign genes in plant cells that are capable of synthesizing specific natural products. We describe a method for the production of transgenic hairy root cultures of peanut (Arachis hypogaea L.) using Agrobacterium rhizogenes R1000, a strain with the binary vector pBI121. We evalua...

The production of sesquiterpene-type phytoalexins with a vetispyradiene skeleton by Hyoscyamus albus hairy roots induced by methyl jasmonate (MeJA) was reported in a previous paper. The production pattern on co-treatment with cupper sulfate and MeJA (CuSO(4)-MeJA) showed a TLC profile differing from that on treatment with MeJA. Thus, we studied the production of phytoalexins on hairy root cultu...

Hairy root cultures were established after inoculation of Genista tinctoria in vitro shoots with Agrobacterium rhizogenes, strain ATCC 15834. In transformed roots of G. tinctoria grown in Schenk-Hildebrandt medium without growth regulators the biosynthesis of isoflavones, derivatives of genistein and daidzein, and flavones, derivatives of luteolin and apigenin, characteristic for the intact pla...

Root extracts of Rauwolfia serpentina have been used for centuries in Ayurvedic medicine as a panacea for a wide variety of physical as well as mental disorders. The potential of Agrobacterium rhizogenes-mediated genetic transformation for the synthesis of phytomolecules of high pharmaceutical value is now well established and documented. Transgenic roots were induced from R. serpentina leaf ex...

Rehmannia glutinosa is a common bulk medicinal material that has been widely used in China due to its active ingredients. Acteoside, one of the ingredients, has antioxidant, antinephritic, anti-inflammatory, hepatoprotective, immunomodulatory, and neuroprotective effects, is usually selected as a quality-control component for R. glutinosa herb in the Chinese Pharmacopeia. The acteoside biosynth...

it is an increased interest in the therapeutic potential of cannabis sativa l. (marijuana) for treatment of multiple sclerosis and hiv neuropathy. because of limitation in cultivation of this plant, an efficient hairy root induction system for cannabis sativa l. was developed in the present study. agrobacterium rhizogenes mediated transformation performed by two different co-cultivation mediums...