In vitro raised shoots of Plumbago rosea L. were infected with A4 strain of Agrobacterium rhizogenes to initiate hairy root formation, which produced 3.0±0.33 hairy roots per incision on explants in 20 d incubation. Southern blot analysis confirmed the integration of T-DNA into the genome of the roots. The hairy roots were cultured on MS agar medium supplemented with 2.0 mg/L BAP to induce the ...

Free radicals play an important role in pathological processes such as aging and developing cancer; hence, natural products inhibit free radical production, and can play an important role in preventing diseases. We aimed to evaluate the scavenging activity of free radicals, reducing power, inhibition of lipid peroxidation and malondialdehyde (MDA) formation, and the antioxidant composition of r...

Hairy root transformation has been widely adapted in plant laboratories to rapidly generate transgenic roots for biochemical and molecular analysis. We present hairy root transformations as a versatile and adaptable model system for a wide variety of undergraduate laboratory courses and research. This technique is easy, efficient, and fast making it an ideal tool for undergraduate teaching. Stu...

Portulaca oleracea L. is a medicinal plant find in Europe and Asia. This plant contains valuable secondary metabolite such as noradrenalin. Naturally the wild plant produce small amount of this secondary metabolites so induction of hairy roots can be useful to increase its production. In our research Agrobacterium rhizogenes strain AR15834 was used to induce hairy roots in the two-week leaf exp...

Salvia miltiorrhiza is an annual plant growing in China, Mongolia, Korea and some other Asian countries. The extract from S. miltiorrhiza roots has been used for supporting healthy cardiovascular and circulatory systems during the last decade. The active constituents of S. miltiorrhiza from different areas vary significantly, and the wild resources are overexploited. To adapt the demand for act...

Background: Trigonella foenum-graecum L. commonly known as fenugreek is a rich source of important medicinal metabolite, i.e. trigonelline. Objective: In this study, hairy roots culture as a novel method for trigonelline production was evaluated. Methods: For optimizing the hairy roots culture of Trigonella foenum-graecum, three strains of Agrobacterium rhyzogenes (ATCC15834, MSU440 and K...

Seeds of Silybum marianum which have been used to treat liver diseases have an active component called silymarin. Hairy root cultures of S. marianum could be able to produce silymarin. There is evidence that the use of elicitors has been an important strategy for improving the production of secondary metabolites. Elicitation is an interesting topic to investigate cell signaling pathway in secon...

Transformed hairy roots had been efficiently induced from the seedlings of Fagopyrum tataricum Gaertn. due to the infection of Agrobacterium rhizogenes. Hairy roots were able to display active elongation with high root branching in 1/2 MS medium without growth regulators. The stable introduction of rolB and aux1 genes of A. rhizogenes WT strain 15834 into F. tataricum plants was confirmed by PC...