This study investigated the effects of homocysteine (Hcy) on angiogenesis in cultured human umbilical vein endothelial cells (HUVEC) and zebrafish embryos. We found that Hcy (50 micromol/L) significantly decreased cell numbers, viability, and induced a G1/S arrest in HUVEC in the presence of adenosine (Ade, 50 micromol/L). Hcy, in combination with Ade, reduced migration and suppressed tube-like...

Proximal tubular epithelial cells are major sites of homocysteine (Hcy) metabolism and are the primary sites for the accumulation and intoxication of inorganic mercury (Hg(2+)). Previous in vivo data from our laboratory have demonstrated that mercuric conjugates of Hcy are transported into these cells by unknown mechanisms. Recently, we established that the mercuric conjugate of cysteine [2-ami...

A push-pull fluorogenic reagent, NBD-SCN, was applied for specific detection of cysteine (Cys) and homocysteine (Hcy). Replacing thiocyanato group with Cys/Hcy increased the push-pull characteristic of the probe and resulted in emission of fluorescence. The fluorescent response of the probe toward Cys/Hcy was significantly higher than toward glutathione and other amino acids. The probe showed a...

Homocysteine (Hcy) is an intermediate of methionine metabolism that, at elevated levels, is an independent risk factor for vascular disease and atherothrombosis. Patients with renal disease, who exhibit unusually high rates of cardiovascular morbidity and death, tend to be hyperhomocysteinemic, particularly as renal function declines. This observation and the inverse relationship between Hcy le...

Many human epidemiologic studies have reported associations between plasma homocysteine (Hcy) concentrations and cardiovascular disease, Alzheimer’s disease, and osteoporosis. However, few studies have examined the relationship between Hcy and disease in dogs. In this study, we investigated the relationship between canine plasma Hcy concentrations and sex, age, breed, size; spay/neuter status, ...

Objective: To study the effect of folate treatment on homocysteine (HCY) levels in patients with Coronary Artery Disease (CAD). We also made an attempt to analyze HCY levels in relation to other risk factors present in these patients. Materials and Methods: The study was a randomised, non-blind clinical trial carried out in 84 angiographically documented CAD patients and 40 controls (angiograph...

OBJECTIVE Hypothyroidism (HO) can induce metabolic dysfunctions related to insulin resistance and dyslipidemia. Our previous studies showed that homocysteine (Hcy) impaired the coronary endothelial function and that Hcy can promote chemokine expression and insulin resistance (IR) by inducing endoplasmic reticulum stress in human adipose tissue and hypothyroid patients. The aim of this study was...

Elevated plasma levels of homocysteine (Hcy), known as hyperhomocysteinemia (HHcy), are associated with osteoporosis. A decrease in bone blood flow is a potential cause of compromised bone mechanical properties. Therefore, we hypothesized that HHcy decreases bone blood flow and biomechanical properties. To test this hypothesis, male Sprague-Dawley rats were treated with Hcy (0.67 g/L) in drinki...

Elevated levels of homocysteine (hcy) form a vascular risk factor. It has been shown that hcy induces cell death in vascular smooth muscle cells (smcs). Recent studies have shown that nadph oxidase (nox)-mediated reactive oxygen species (ros) play a role in hcy-induced apoptosis in cardiomyocytes and endothelial cells. In this study we have analyzed the role of hcy on the different nox isoforms...

OBJECTIVE We previously reported that homocysteine (Hcy) inhibits endothelial cell (EC) growth and promotes vascular smooth muscle cell (VSMC) proliferation. This study characterized and directly compared Hcy transport in cultured human aortic ECs (HAECs) and smooth muscle cells (HASMCs). METHODS AND RESULTS Hcy (10 micromol/L) was transported into both cell types in a time-dependent fashion ...