نتایج جستجو برای: hek 293
تعداد نتایج: 11391 فیلتر نتایج به سال:
مقدمه: گلیکوپروتئین 3tim (3t-cell immunoglobulin and mucin domain) یکی از نشانگرهای سطح سلولی سلول های 1th (1t helper) است که در بسیاری از بیماری های مرتبط با سیستم ایمنی، تغییر بیان دارد. این مطالعه، با هدف بررسی و افزایش بیان پروتئین 3tim در سطح سلول t293 انجام شد. روش ها: کاست بیانی 3tim از روی پلاسمید 67m-2682w-ex با استفاده از پرایمرهای دارای جایگاه آنزیمی nhei و mlui تکثیر و در جایگاه های...
Aim: Dexmedetomidine; it is widely used in anesthesia and intensive care. We aimed to examine compare the cytotoxic, reactive oxygen species (ROS) apoptotic effects of dexmedetomidine on kidney cells (Hek-293) vitro at two different high cumulative doses. Material Methods: The half-maximum inhibitory concentration (IC50) dose Hek-293 was determined using 3-[4,5-dimethylthiazol-2yl]-2,5-diphenyl...
Activation of PKR (double-stranded-RNA-dependent protein kinase) by DNA plasmids decreases translation, and limits the amount of recombinant protein produced by transiently transfected HEK (human embryonic kidney)-293 cells. Co-expression with Ebola virus VP35 (virus protein 35), which blocked plasmid activation of PKR, substantially increased production of recombinant TPL-2 (tumour progression...
BACKGROUND Ginsenosides are the main pharmacological components of Panax ginseng root, which are thought to be primarily responsible for the suppressing effect on oxidative stress. METHODS 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and oxygen radical absorption capacity were applied to evaluate the antioxidant activities of the ginsenosides. Human embryonic kidney 293 (HEK-293)...
The four highly conserved intracellular tyrosine residues of the P2X(1) ion channel were mutated into phenylalanine. Simultaneous electrophysiological and calcium measurements in transfected human embryonic kidney (HEK 293) cells indicated that Y362F and Y370F mutants were non-functional, despite their proper plasma membrane expression. The Y16F and Y363F mutants retained 2.2% and 26% of the wi...
Sialylation of N-glycans associated with recombinant human acetylcholinesterase (rHuAChE) has a central role in determining its circulatory clearance rate. Human embryonal kidney 293 (HEK-293) cells, which are widely used for the expression of recombinant proteins, seem to be limited in their ability to sialylate overexpressed rHuAChE. High-resolution N-glycan structural analysis, by gel permea...
An important question in the pathophysiology of dominantly inherited diseases, such as channelopathies, is the level of expression of the mutant protein. In our study, we address this issue by comparing the gating defects of two human muscle NaF channel mutants (R1448C and R1448P) causing paramyotonia congenita in native muscle specimens from two patients with those of the same mutant recombina...
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