Costello syndrome (CS) patients suffer from a very high 10% incidence of embryonal rhabdomyosarcoma (ERMS). As tools to discover targeted therapeutic leads, we used a CS patient-derived ERMS cell line (CS242 ERMS) harboring a homozygous p.G12A mutation in HRAS, and a control cell line derived from the same patient comprising non-malignant CS242 fibroblasts with a heterozygous p.G12A HRAS mutati...

BACKGROUND Currently, cationic liposome has become the commonly used vehicles for gene transfection. Furthermore, one of the most significant steps in microRNAs expression studies is transferring microRNAs into cell cultures successfully. In this study we aim to approach the feasibility of transfection of cervical cancer cell lines mediated by liposome and to obtain the optimized transfection c...

mycoplasma and ureaplasma species are the most serious contaminants of cell cultures and this remains one of the major problem encountered in biological research using cell cultures. the aim of this study was to investigate the rate of mycoplasma and a total of 100 different cell culture specimens including, r228, hela, vero, mdck, l20, rd and hep2 were collected. then the specimens were invest...

background: most cancer cells become resistant to anti-cancer agents. in the last few years, a new approach for targeted therapy of human cancer has been developed using immunotoxins which comprise both the cell targeting and the cell killing moieties. methods: in the present study, the recombinant shiga toxin a1 subunit fused to human granulocyte-macrophage colony stimulating factor (a1-gm-csf...

in this syudy a continuous bovine kidney cell line derived from a primary bovine kidney cells was established for the first time in iran. the cells were originating from two-day-old normal male calf of holstein breed. the cell cultures were continuously passaged following complete proliferation of primary cells. the specific properties or characteristics of the cell were defined using cytogene...

The objective of this study was to observe the apoptosis-inducing effect and mechanism of baicalin on human cervical cancer HeLa cells. The inhibitory effect of baicalin on the growth of HeLa cells was measured by MTT assay, and cell proliferation and migration was analyzed by cell scratch assay. Morphological changes of apoptotic cells were viewed by the light microscope and electron microscop...

The objective of this study was to observe the apoptosis-inducing effect and mechanism of baicalin on human cervical cancer HeLa cells. The inhibitory effect of baicalin on the growth of HeLa cells was measured by MTT assay, and cell proliferation and migration was analyzed by cell scratch assay. Morphological changes of apoptotic cells were viewed by the light microscope and electron microscop...

Abstract The aim of study was to determine any anticancer potential from Avicennia marina leaf extract compare taurine on cytotoxic and anti-proliferation activities against HeLa cervical cancer cell line by using the MTT method (3-(4, 5-dimethylthiazol-2-yl))-2, 5-diphenyltetrazolium bromide). results indicated that methanol extraction A. as well did have effects with IC50 values 321 ppm 603 1...

A squamous cell carcinoma tumor cell line, COLO 227, derived from a metastatic tumor in a Caucasian male, produces both parathyroid hormone and carcinoembryonic antigen. A chromosome mode of 106 predominated and the X and Y chromosomes were retained. Seven marker chromosomes were identified. Cytogenetic analysis revealed an isochromosome 8 marker similar to a HeLa cell line marker and an isochr...

A series of novel amide derivatives of cembranoid neocrotocembraneic acid were designed and synthesized. The antiproliferative activities of these derivatives were evaluated against three human tumor cell lines (the human cervical cancer cell line HeLa, chronic myeloid leukemia cell line K562 and leukemia multidrug-resistant cell line K562/A02). Some of the synthesized compounds exhibited moder...