نتایج جستجو برای: hela cells
تعداد نتایج: 1385517 فیلتر نتایج به سال:
Gap junctional communication modulates intercellular calcium signaling in many cell types. We have investigated whether gap junctional communication modulates calcium oscillatory behavior of cells responding to an agonist. Extracellular UTP induced calcium oscillations in 70% of HeLa cells cultured in monolayer, and neighboring cells oscillated independently of each other. In HeLa cell transfec...
1. We have recently re-tested the STR fingerprinting data in our paper (Int J Biol Sci 2011; 7:892-901). DNA fingerprinting analysis was performed using genomic DNA from hsNSCs (passage 22); T1 cells (passages <50 and >150) and HeLa cells by GeneMapper ID v3.2 (Applied Biosystems, Foster City, CA) for human STR markers. There are two loci of heterozygosity CSF1PO (9, 10) and D3S1358 (15, 18) in...
BACKGROUND/AIM The claudin family of proteins are key constituents of tight junctions and the aberrant expression of these proteins can contribute to de-stabilisation of tight junctions and thus to loss of cell polarity and cohesion. Increased expression of claudin-1 and claudin-7 has been observed in pre-invasive cervical lesions and cervical carcinomas. The present study attempted to assess t...
introduction: the aim of this study was to determine a proper cell line for adherance of helicobacter pylori and a suitable method for attachment of this bacteria to cell lines in vitro. material and methods: a total of 22 h.pylori strains,isolated from the antral biopsies of 49 patients with dyspepsia,gastritis,gastric ulcer,duodenal ulcer, etc., were evaluated by elisa(upr)to determine the di...
Propyl gallate (PG) used in processed food and medicinal preparations has been shown to induce cell death in normal and cancer cells. The inhibition of proteasome function has emerged as a useful strategy to maneuver apoptosis. Here, we investigated the combined effects of PG and MG132 (a proteasome inhibitor) on HeLa cells in relation to cell growth, cell death, reactive oxygen species (ROS) a...
Extracts derived from Viscum album have been shown to kill cancer cells in vitro. Some studies have noted that different species of this plant collected from around the world displayed cytotoxic effects in different extents. In the present study, we evaluated the effects of Iranian mistletoe extracts on five cancer cell lines. Plants growing on hornbeam tree (Carpinus...
Two gap junction proteins, connexin43 (Cx43) and connexin45 (Cx45), are coexpressed in many cardiac and other cells. Homomeric channels formed by these proteins differ in unitary conductance, permeability, and regulation. We sought to determine the ability of Cx43 and Cx45 to oligomerize with each other to form heteromeric gap junction channels and to determine the functional and regulatory pro...
Several variants resistant to 1.8 x 10(-4) M DL-methotrexate (MTX) have been isolated from the human cell lines HeLa BU25 and VA2-B by exposing them to progressively increasing concentrations of the drug. A striking variability of phenotype and chromosome constitution was observed among the different variants. All resistant cell lines exhibited a greatly increased dihydrofolic acid reductase (D...
Aspirin and other nonsteroidal anti-inflammatory drugs show efficacy in the prevention of cancers. It is known that they can inhibit cyclooxygenases, and some studies have shown that they can induce apoptosis. Our objective in this study was to investigate the mechanism by which aspirin exerts its apoptosis effects in human cervical cancer HeLa cells. The effect of aspirin on the gene expressio...
The horse-adapted virulent Bucyrus (VB) strain of equine arteritis virus (EAV) established persistent infection in high-passage-number human cervix cells (HeLa-H cells; passages 170 to 221) but not in low-passage-number human cervix cells (HeLa-L cells; passages 95 to 115) or in several other cell lines that were evaluated. However, virus recovered from the 80th passage of the persistently infe...
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