External signals that are mediated by specific receptors determine stem cell fate. The thrombin receptor PAR1 plays an important role in haemostasis, thrombosis and vascular biology, but also in tumor biology and angiogenesis. Its expression and function in hematopoietic stem cells is largely unknown. Here, we analyzed expression and function of PAR1 in primary hematopoietic cells and their leu...

Blood cells transplantation is largely replacing bone marrow transplantation because engraftment is more rapid. This accelerated engraftment is thought to be mediated by relatively mature committed hematopoietic progenitor cells. Herein, we have used a modified rhodamine (Rho) staining procedure to identify and purify Rho+/++ (dull/bright) and Rho- (negative) subpopulations of hematopoietic pro...

Stem cells are emerging as an attractive option because of their intrinsic ability vectors for drug delivery to diseased tissues. The term stem or was first introduced by a Russian histologist, Alexander Maksimov (1874-1928). purpose this study is describe the research and development cell applications stroke. This literature (review article) regarding (stem cells) in stroke which has been disc...

Mouse hematopoietic stem cells can be identified and enriched from populations of normal bone marrow cells by immunofluorescent labeling of cell surface molecules followed by flow cytometric separation. We show here that the majority of hematopoietic stem cell activity, as defined by long-term competitive repopulation of irradiated animals and by a secondary transplant assay for spleen colony-f...

OBJECTIVES Costimulatory gene polymorphisms have been proposed to affect the hepatitis B virus pathogenesis by affecting regulation of immune responses. The association between costimulatory molecule gene polymorphisms including CTLA4, PD.1, ICOS, and CD28 with hepatitis B virus infection has been evaluated in hematopoietic stem cell transplant patients. MATERIALS AND METHODS In a cross-secti...

objective: receptor activator of nuclear factor-kappa b ligand (rankl) appears to be an osteoclast-activating factor, bearing an important role in the pathogenesis of multiple myeloma. some studies demonstrated that u-266 myeloma cell line and primary myeloma cells expressed rank and rankl. it had been reported that the expression of myeloid and monocytoid markers was increased by co-culturing ...

Hematopoietic stem progenitor cells are the source for the entire hematopoietic system. Studying gene expression in hematopoietic stem progenitor cells will provide information to understand the genetic programs controlling early hematopoiesis, and to identify the gene targets to interfere hematopoietic disorders. Extensive efforts using cell biology, molecular biology, and genomics approaches ...

Stem cell therapies may be valuable in treatment of inflammatory bowel disease (IBD). Here we focus on two very different types of stem cells - hematopoietic stem cells and mesenchymal stem cells. Myeloablation and hematopoietic stem cell transplantation alter host immune response by reconstituting the recipient's blood cell lines with donor cells. Autologous hematopoietic reconstitution may "r...

Adult hematopoietic tissue-derived stem cells are primarily used for hematopoietic reconstitution in patients with malignant lympho-hematopoietic disorders undergoing stem cell transplantation. Their therapeutic use for solidorgan tissue repair such as cardiac tissue is a novel treatment strategy. Besides hematopoietic stem cells (HSCs), mesenchymal stem cells (MSCs), endothelial progenitor cel...

Studies on hematopoiesis have focused on the function and composition of human bone marrow stroma. Stroma function gives hematopoietic stem cells the microenvironment appropriate for self-renewal and/or prompt differentiation into hematopoietic progenitor cells, then into terminal specialized cells. Human bone marrow stroma has been dissected into hematopoietic and nonhematopoietic components. ...