The Huynh electronic parameter (HEP) is a modern alternative method to determine ligand donor strengths by 13C NMR spectroscopy of metal NHC complexes containing the interest. Using HEP, properties numerous classical Werner-type and organometallic ligands have been evaluated thus far. Moreover, it has extended HEP2, which provides means measure bidentate chelators. This Highlight Review covers ...

A potent new enzyme-antibody conjugate system for amplifying cytotoxicity was tested in a well-defined model of hapten [2,4,6-trinitrophenyl (TNP)]-substituted tumor cells (HEp2) and purified anti-hapten antibody. Brief treatment of TNP-HEp2 cells with low concentrations (0.05 to 0.74 micrograms/ml) of antihapten antibody-alcohol dehydrogenase conjugate (Ab-ADH) followed by culture in complemen...

A solution hybridization/RNase protection assay with riboprobes was developed to quantitate apolipoprotein mRNA concentrations. Previously, radiolabeled DNA probes have been used in solution hybridization/S1 nuclease protection assays for this purpose. The new assay requires less time for probe preparation and hybridization compared to previous assays. In addition, the vector used for riboprobe...

objective: as we are approaching the global eradication of wild poliovirus, laboratory surveillance of poliovirus by the gold standard cell culture method becomes increasingly important. as there is a lot of concern about accurate and sensitive detection of imported wild and vaccine derived polioviruses (vdpvs) in polio-free countries, in this study we assessed and compared the sensitivity of t...

CD147, also named extracelluar matrix metalloproteinase inducer (EMMPRIN), is a member of the immunoglobulin family and a glycoprotein enriched on the surface of tumor cells, which promotes invasion, metastasis, growth and survival of malignant cells, and is known to confer resistance to some chemotherapeutic drugs. To determine the possible role of CD147 in the invasive properties of laryngeal...

Using biological medium, such as plant extract for the biosynthesis of nanoparticles is an ecofriendly and emerging scientific trend. On this back drop the present study focused on the biosynthesis of silver nanoparticles using Cissus quadrangularis and evaluation of its antimicrobial and anticancer potentials. The biosynthesized nanoparticle was characterized by UV-VIS spectroscopy, EDAX and T...

AIM To explore kinetic changes in the gene expression profile during radiation-induced mitotic catastrophes. MATERIALS AND METHODS Gene expression changes were measured in HPV-infected HeLa Hep2 tumor cells following exposure to 5 Gy of ionizing radiation ((60)Co). Signaling pathways were explored and correlated to the biological responses linked to mitotic catastrophe. RESULTS Following ir...

In this paper we will summarize the activities carried out in the last two years in the MIVIA Lab: exact and inexact graph matching, object tracking and video analysis based on semantic web technologies, HEp2 cells classification, gender recognition and people counting.

background and objective: in addition to the use of chemicals in food, pharmaceutical, cosmetic and hygiene industries, use of herbal and natural compounds have also increased. it seems natural ingredients compared with the chemical components show less side effects, such as mutagenicity and carcinogenesis however, their use can also be associated with side effects and toxicity. some significan...