Concentrative nucleoside transporter 2 (CNT2) (encoded by the SLC28A2 gene) transports various antiviral or antitumor purine nucleoside analogs to be involved in their pharmacokinetics and pharmacological actions. The results of our study showed that mouse hepatocytes hardly expressed CNT2 mRNA and no CNT2-dependent nucleoside uptake was observed, while rat hepatocytes exhibited high CNT2-depen...

Purpose: To investigate the therapeutic effect and underlying mechanism of hypaphorine in a cellular model non-alcoholic fatty liver disease (NAFLD).Methods: Palmitic acid (PA) was used to induce NAFLD phenotype hepatocytes. Cell viability apoptosis were evaluated by CCK-8 flow cytometry assays. Inflammatory response measured enzyme-linked immunosorbent assay (ELISA). The on lipid accumulation ...

In order to explore the role of forkhead box protein O1 (FoxO1) in lipid metabolism and cell proliferation, goose primary hepatocytes were isolated incubated with insulin or PI3K-Akt-mTOR pathway dual inhibitor NVP-BEZ235, then transfected FoxO1 interference plasmid. The related parameters proliferation measured. results firstly showed that increased intracellular TG lipids concentration (P < 0...

Partial hepatectomy (PH) results in the persistent drop of the specific activity of glutamine synthetase (GS) (EC 6.3.1.2). This drop correlates with the reduced proportion of GS+ hepatocytes and with the reduced GS+ area surrounding the central veins such that GS+ hepatocytes are arranged in a single cell layer only. Cultivation of hepatocytes isolated at various times after PH revealed consid...

Oxidative stress has been associated with valproic acid (VPA) treatment, and mitochondrial dysfunction has been implicated in the pathogenesis of VPA-idiosyncratic hepatotoxicity. The present study investigated the effect of VPA and the role of GSH on oxidative stress, mitochondrial membrane potential, and toxicity in freshly isolated rat hepatocytes. Hepatocytes were isolated from Sprague-Dawl...

Although embryonic stem (ES) cell-derived hepatocytes have the capacity for liver engraftment and repopulation, their in vivo hepatic function has not been analyzed yet. We aimed to determine the metabolic function and therapeutic action of ES cell-derived hepatocytes after serial liver repopulations in fumaryl acetoacetate hydrolase knockout (Fah(-/-)) mice. Albumin expressing (Alb(+)) cells w...

PURPOSE The major problem in producing artificial livers is that primary hepatocytes cannot be cultured for many days. Recently, 3-dimensional (3D) printing technology draws attention and this technology regarded as a useful tool for current cell biology. By using the 3D bio-printing, these problems can be resolved. METHODS To generate 3D bio-printed structures (25 mm × 25 mm), cells-alginate...

Morphologically and functionally intact human hepatocytes were isolated from small liver biopsy samples weighing about 1-2 g by initial digestion with collagenase followed by repeated digestions with trypsin. The usual yield of hepatocytes was greater than 1 x 10(7) cells per g of liver sample and cell viability, as judged by dye exclusion test, was routinely over 90%. The isolated human hepato...

Early phase drug development relies on primary human hepatocytes for studies of drug metabolism, cytotoxicity, and drug-drug interactions. However, primary human hepatocytes rapidly lose metabolic functions ex vivo and are refractory to expansion in culture and thus are limited in quantity. Hepatocytes derived from human pluripotent stem cells (either embryonic stem (ES) or induced pluripotent ...

The number of approved peptide therapeutics has increased significantly in recent years. Peptide have many advances over small molecule drugs, such as higher affinity to target and lower toxicity profiles. Although peptide-like drugs are mainly metabolized/catabolized the body for smaller peptides amino acids, metabolite identification still an essential part their development, especially if st...