Glucagon and dibutyryl cyclic AMP inhibited glucose utilization and lowered fructose 2,6-bisphosphate levels of hepatocytes prepared from fed chickens. Partially purified preparations of chicken liver 6-phosphofructo-1-kinase and fructose 1,6-bisphosphatase were activated and inhibited by fructose 2,6-bisphosphate, respectively. The sensitivities of these enzymes and the changes observed in fru...

Livers from human fetuses between the 16th and 24th weeks of gestation were dissociated by successive dispase and collagenase digestion followed by two cycles of low-speed differential centrifugation. This improved method recovered approximately 1 X 10(7) cells (90% hepatocytes and 90% viable cells) from 4 g of liver tissue. These hepatocytes were set into primary culture and monolayer granular...

conclusions hepatocyte isolation can be performed as the main and initial step for cell transplantation from unused/rejected liver. it is the first experience in iran. results highly quality hepatocytes with high viability and low contamination were isolated. the mean viability was 71.8% ± 21.7. in the first 4 cases microbial contamination by staphylococci, diphtheroid and klebsiella was detect...

Abstract Background Domestic cats rarely develop hepatocellular carcinoma. The reason for the low prevalence is unknown. Reductions in ploidy have been associated with hepatic carcinogenesis. Recent work mice has shown that livers more polyploid hepatocytes are protected against development of Hepatocyte domestic cat not evaluated. We hypothesized would be reduced peri-tumoral and neoplastic co...

Background: Hepatocytes transplantation (HCTx) is an alternative method to orthotopic liver transplantation used in treatment of several liver injuries. It can be used either fresh or as encapsulated hepatocytes. Expression of Hep Par 1, CK7 and Albumin were performed to identify and analyze the transplanted isolated adult hepatocytes in hamsters spleens. Aim: This work aimed to study the expre...

Induction of cytochromes P450 (P450s) by drugs can lead to drug-drug interactions. Primary hepatocytes have been reported to retain inducible P450s. To optimize the use of primary hepatocytes for predicting induction of P450 (CYP 3A and 2B) expression in vivo, both culture conditions and expression of induction potentials were investigated. In rat hepatocytes, basal CYP 3A1/2 expression was bet...

Immortalized p19(ARF) null hepatocytes (MIM) feature a high degree of functional differentiation and are susceptible to transforming growth factor (TGF)-beta driven growth arrest and apoptosis. In contrast, polarized MIM hepatocytes expressing hyperactive Ha-Ras continue proliferation in cooperation with TGF-beta, and adopt an invasive phenotype by executing an epithelial to mesenchymal transit...

Cryopreserved hepatocytes are often used as a convenient tool in studies of hepatic drug metabolism and disposition. In this study, the expression and activity of drug transporters in human and rat fresh and cryopreserved hepatocytes was investigated. In human cryopreserved hepatocytes, Western blot analysis indicated that protein expression of the drug uptake transporters [human Na(+)-taurocho...

The objective of this study was to isolate hepatocytes of the proximal half (Zones 1 and 2) or distal half (Zones 2 and 3) of the liver acinus. The zonal origin of the isolated hepatocytes was recognized by: the presence in hepatocytes of a fluorescent marker, acridine orange, selectively delivered to either the proximal or the distal half of the acinus by in situ perfusion prior to cell isolat...