To analyze mechanisms of liver repopulation, we transplanted normal hepatocytes into syngeneic rats deficient in dipeptidyl peptidase IV activity. When isolated hepatocytes were injected into splenic pulp, cells promptly migrated into hepatic sinusoids. To examine whether transplanted hepatocytes entered liver plates and integrated with host hepatocytes, we analyzed sharing of hepatocyte-specif...

          Leishmaniasis is caused by an intracellular parasite . It endemic in Asia, Africa, the Americas, and Mediterranean region. Worldwide, 1.5 to 2 million new cases occur each year .The histological study of liver tissue white laboratory mice (Mus musculus) infected with L. major a showed The presence severe steatosis hepatocytes Hepatocyte deg...

The effect of antiviral therapy during Hepatitis C Virus (HCV) infection is the focus this study. HCV destroys healthy hepatocyte cells in human liver, causing cirrhosis and hepatocellular carcinoma. We introduce a cell-population model representing long-term dynamics response to drug therapies. proliferation existing can create system. Such models are based on susceptible hepatocytes, infected...

amodiaquine is an antimalarial drug used in the prophylaxis and treatment of this disease. however, hepatotoxicity as a life‑threatening adverse effect is associated with its clinical use. we evaluated amodiaquine-induced toxicity in isolated rat hepatocytes as an in vitro model for studying drug‑induced hepatotoxicity. this study attempts to investigate the protective effects of taurine and n-...

As an intracellular pathogen, the reproduction of hepatitis B virus (HBV) depends on occupancy host metabolism machinery. Here we test a hypothesis if HBV may govern biosynthesis to achieve productive reproduction. To this hypothesis, set up affinity purification screen for factors that interact with large viral surface antigens (LHBS). This identified pyruvate kinase isoform M2 (PKM2), key reg...

Primary cultures of adult mouse hepatocytes are shown here to reexpress differentiated hepatocyte features following treatment with 2% DMSO and 10(-7) M glucagon. To examine the roles of gap junctional communication during hepatocyte growth and differentiation, we have compared treated and untreated hepatocytes from connexin (Cx)32-deficient [Cx32 knockout (KO)] and wild-type mice. In untreated...

We have investigated the effect of a therapeutic agent for liver disease, Laennec, on the GOT leakage from freshly isolated and primary cultured rat hepatocytes which were treated with CCl4. By treatment with Laennec together with CCl4, the GOT leakage from isolated hepatocytes increased and that from cultured hepatocytes decreased, compared to those incubated only with CCl4. The results sugges...

BACKGROUND Many studies have suggested that the immune response may play a crucial role in the progression of hepatocellular carcinoma (HCC). Therefore, our aim was to establish a (i) functional culture of primary human tumor hepatocytes and non-tumor from patients with hepatocellular carcinoma (HCC) and (ii) a co-culture system of HCC and non-HCC hepatocytes with autologous peripheral blood mo...

BACKGROUND In farm animals, there is no suitable cell line available to understand liver-specific functions. This has limited our understanding of liver function and metabolism in farm animals. Culturing and maintenance of functionally active hepatocytes is difficult, since they survive no more than few days. Establishing primary culture of hepatocytes can help in studying cellular metabolism, ...

Freshly isolated hepatocytes are considered the gold standard for in vitro studies of hepatic drug disposition. To ensure a reliable supply of cells, cryopreserved human hepatocytes are often used. ABC-superfamily drug efflux transporters are key elements in hepatic drug disposition. These transporters are often considered lost after isolation of hepatocytes. In the present study, the expressio...