Antigens isolated from herpes simplex virus type 1, herpes simplex virus type 2, or cytomegalovirus-transformed hamster cells were tested against 66 sera from non-cancer individuals or patients with different types of cancer. By use of the microcomplement fixation procedure to quantify all antigen-antibody interactions, it was observed that 94% (p less than 0.001) of all sera from patients with...

Viruses as obligate intracellular parasites use host cell proteins to ensure efficient replication and spread. Cellular proteins are required for several stages of a virus life cycle. Here, we identify BAG3, a co-chaperone, as a regulator of herpes virus immediate early gene expression. We report that a herpes simplex virus lacking the gene encoding a potent transcriptional activator, ICP0, is ...

background: oncolytic herpes simplex virus (ohsv) vectors lacking γ34.5 has demonstrated anticancer effects without affecting normal cells. therefore, they are considered as ideal templates to construct efficient vectors for tumor targeting and cancer gene therapy. herein, we reported construction of three single/dually-labeled recombinant hsv-1 vectors, which provide an opportunity to replace ...

background: the herpes simplex virus (hsv) ul41 gene product, virion host shutoff (vhs) protein, mediates the rapid degradation of both viral and cellular mrna. this ability suggests that vhs protein can be used as a suicide gene in cancer gene therapy applications. the recent reports have shown that the degradation of cellular mrna during herpes simplex infection is selective. rna containing a...

Subjects with oral herpes lesions at the time of serum sampling had higher-efficiency antibody (higher proportion of neutralizing antibody as determined by plaque reduction, compared with total antibody as detected by radioimmunoassay) to herpes simplex virus type 1 (HSV-1) than did subjects with no lesions at the time of serum sampling. These higher-efficiency sera also had higher antibody tit...

Herpes simplex virus is most probably maintained in the ganglion neurons of the peripheral nervous system of humans in a latent form that can reactivate to produce recurrent disease. As an approximation of this cell-virus interaction, we have constructed a herpes simplex virus latency in vitro model system using human fetus sensory neurons as the host cell. Human fetus neurons were characterize...

During 1984 the British Co-operative Clinical Group undertook a survey of genitourinary medicine clinics in the United Kingdom to assess the availability of laboratory diagnostic facilities for Chlamydia trachomatis and herpes simplex virus. Of the 115 clinics assessed, 68% (78) had adequate facilities for culture for C trachomatis and 87% (100) for herpes simplex virus.

We presented four cases diagnosed with Herpes simplex virus encephalitis of those treated in our clinic in 2006 one of whom died, one recovered without any sequels. Two cases were left with advanced motor mental sequel. In this paper, clinical and laboratory findings along with diagnosis and treatment of Herpes simplex virus encephalitis were discussed.

background: sodium valproate, an anticonvulsant drug, is reported to stimulate human immunodeficiency virus type 1 and human cytomegalovirus replication. since epileptic patients undergoing sodium valproate therapy may suffer from various virus infections, the effect of this drug on replication of viruses especially those affecting neuronal tissues such as herpes simplex virus type 1 is worthy ...

Cord serum samples treated with staphylococcus protein A and an immune affinity column to remove immunoglobulin G were used to establish a seronegative range for herpes simplex virus and cytomegalovirus. No seroconversions or increased heterologous antibody levels to herpes simplex virus or cytomegalovirus were found in live varicella vaccine recipients.