Analysis of intraspecies diversity in wheat and barley genomes identifies breakpoints of ancient haplotypes and provides insight in the structure of diploid and hexaploid Triticeae gene pools Abstract A large number of wheat and barley varieties have evolved in agricultural ecosystems since domestication. Because of the large, repetitive genomes of these Triticeae crops, sequence information is...

Insertional mutagenesis and gene silencing are efficient tools for the determination of gene function. In contrast to gainor lossof-function approaches, RNA interference (RNAi)-induced gene silencing can possibly silence multigene families and homoeologous genes in polyploids. This is of great importance for functional studies in hexaploid wheat (Triticum aestivum), where most of the genes are ...

During meiosis, homologous chromosomes synapse and recombine at sites marked by the binding of the mismatch repair protein MLH1. In hexaploid wheat, the Ph1 locus has a major effect on whether crossover occurs between homologues or between related homoeologues. Here we report that--in wheat-rye hybrids where homologues are absent--Ph1 affects neither the level of synapsis nor the number of MLH1...

We report the results of a study on the effectiveness of Cot filtration (CF) in the characterization of the gene space of bread wheat (Triticum aestivum L.), a large genome species (1C = 16,700 Mb) of tremendous agronomic importance. Using published Cot data as a guide, 2 genomic libraries for hexaploid wheat were constructed from the single-stranded DNA collected at Cot values > 1188 and 1639 ...

Pseudomonas syringae is one of the most widespread plant pathogens that can cause significant damage to crop plantations. Here, we announce a noncontiguous finished genome sequence of Pseudomonas syringae pv. syringae strain SM, isolated from hexaploid wheat. The genome sequence revealed the smallest described complement of type III effectors.

The domestication of wheat was instrumental in spawning the civilization of humankind, and it occurred through genetic mutations that gave rise to types with non-fragile rachises, soft glumes, and free-threshing seed. Wild emmer (Triticum turgidum ssp. dicoccoides), the tetraploid AB-genome progenitor of domesticated wheat has genes that confer tenacious glumes (Tg) that underwent genetic mutat...

BACKGROUND In higher plants, inorganic nitrogen is assimilated via the glutamate synthase cycle or GS-GOGAT pathway. GOGAT enzyme occurs in two distinct forms that use NADH (NADH-GOGAT) or Fd (Fd-GOGAT) as electron carriers. The goal of the present study was to characterize wheat Fd-GOGAT genes and to assess the linkage with grain protein content (GPC), an important quantitative trait controlle...

The utility of mining DNA sequence data to understand the structure and expression of cereal prolamin genes is demonstrated by the identification of a new class of wheat prolamins. This previously unrecognized wheat prolamin class, given the name δ-gliadins, is the most direct ortholog of barley γ3-hordeins. Phylogenetic analysis shows that the orthologous δ-gliadins and γ3-hordeins form a dist...

A total of 4,131 expressed sequence tags (ESTs) were selected from wheat EST database (http://wheat.pw.usda.gov/cgi-bin/westsql/est_lib.cgi.) to identify genic regions differing at structural and functional level between durum wheat (tetraploid genome, AABB) and bread wheat (hexaploid genome, AABBDD) cultivars in response to salt stress. Selected ESTs from salt stressed Triticum aestivum cDNA l...