A novel, simple and accurate fingerprint method was developed using high-performance liquid chromatography-photodiode array detection (HPLC-DAD) for the quality control of Qianghuo, a Tibetan folk and Chinese herbal medicine used as a diaphoretic, an antifebrile and an anodyne. For the first time, the feasibility and advantages of employing chromatographic fingerprint were investigated and demo...

We describe an HPLC method for quantifying creatinine, separating the analyte from other compounds in serum and urine by cation-exchange chromatography and measuring its absorbance at 234 nm. The precision of the method (CV) varied from 2.9% (mean creatinine concentration, 31 mumol/L) to 1.7% (361 mumol/L) within a series of assays and from 3.9% (34 mumol/L) to 2.4% (391 mumol/L) between series...

A three-dimensional high-performance liquid chromatography (HPLC) method is described for the determination of cyclosporine A in human skin. The method allows to determine at least 3 ng cyclosporine A in 10 mg skin (total sample amount needed) and is suitable for cyclosporine A level monitoring in the skin of psoriatic patients.

A chemometric tool based on the Function of Mutual Information (FUMI) theory can provide a relative standard deviation (RSD) without repetitive measurements in high-performance liquid chromatography with electrochemical detection (HPLC-ECD). Two parameters: precision (= information content) phi and efficiency (= information content/time) theta, which were calculated from predicted RSD based on ...

High-performance liquid chromatography is a technic that has recently been applied to the diagnosis of hemoglobinopathies and thalassemias. Its advantages over other methods include increased sensitivity, resolution and simplicity, as well as speed. In this report, the authors present an HPLC procedure that uses a weak cation exchange column and a gradient elution system for the diagnosis of he...

the fast depletion of petroleum crude oil reserves and the serious consideration of environmental issues make the implementation of sustainable energy sources a crucial issue worldwide. biofuel derived from vegetable oils is receiving a great attention as one of the most suitable and logical alternatives of fossil fuels. therefore, the production of petroleum-like fractions from nonedible oil e...

A convenient method for the separation of molecular species of complex lipids such as sphingomyelin and ceramide by argentation high performance liquid chromatography (HPLC) is described for the first time. Molecular species separation of these complex lipids was achieved for quantitative analysis and preparative work by the combined use of argentation and reversed-phase HPLC.-Smith, M., P. Mon...

We have carried out a single-dose comparison of three different dextromethorphan cough mixtures in 10 healthy human volunteers. Dextromethorphan was administered in a single dose of 60 mg in random order. The concentrations of dextromethorphan and its main metabolite, dextrorphan, were determined from the plasma samples using high performance liquid chromatography. The concentrations of dextror...

Monomeric and oligomeric proanthocyanidins present in a range of plant-derived foods and beverages were separated by degree of polymerization and identified using a modified normal-phase high-performance liquid chromatography (HPLC) method coupled with on-line mass spectrometry (MS) analysis using an atmospheric pressure ionization electrospray chamber. In addition, ultraviolet (UV) and fluores...

In the most application of nozzle with gas-liquid two-phase flow, the quality of mixture in exhaust of nozzle is the most important parameter as well as the flow velocity. On the other hand, in some industrial application, such as water injection in forced induction (turbocharged or supercharged) internal combustion engine the spray quality is the main goal of designing. In this case and for im...