experiment was conducted to evaluate the effect of l-glutamine on coated sperm freezing by using four rams. semen was collected by artificial vagina contact with a tube containing tris-fructose-egg yolk 15%. samples were pooled and centrifuged. after removing supernatant and diluting pellet, samples were split into 5 equal part and added 0, 40, 80, 120 and 160 mm l-glutamine. samples were froze...

Maxicircle DNA has been isolated from the kinetoplast DNA of Leishmania tarentolae culture forms by bouyant separation in CsCl in the presence of the A + T-binding dye Hoechst 33258, after liberation from the kinetoplast DNA network by cleavage with the single-hit restriction endonuclease EcoRI. The purified linearized maxicircle DNA has a density in CsCl of 1.681 g/cm3 (79% A + T) and a molecu...

A sensitive fluorimetric assay based on DNA binding of the fluorescent dye Hoechst 33258 was developed to quantitate cell numbers in microtiter plates. The cells were fixed and incubated with the dye solution. The bound dye was extracted with ethanol and fluorescence was measured in a spectrofluorimeter. The method is sensitive for as little as a thousand cells and is particularly useful for th...

To explore the impact of the new derivatives cyclovirobuxine D (CBV-D) model of alcohol-induced injury in PC12 cells. The model of alcohol-induced injury in PC12 cells, new derivatives of the cyclovirobuxine D PC12 cells by MTT assay, Hoechst 33258 staining and lactate dehydrogenase (LDH) activity was measured. The new D derivatives cyclovirobuxine inhibit the damage caused by alcohol PC12 cell...

The influence of antivirals, such as rimantadine, ribavirine and triazavirine on influenza virus replication in human cell cultures was evaluated. All the antivirals inhibited viral nucleoprotein NP synthesis. The strongest effect was shown for ribavirine in lung carcinoma A-549 cells and endothelial ECV-304 cells. Hoechst-33258 staining revealed induction of apoptosis in all the cell lines. Ri...