Background: Spinal cord injury (SCI) occurs most often to young men at the peak of their reproductive health. Only 10% of SCI men can father children without medical assistance due to potential impairments in ejaculation and sperm quality. Objective: The main objective of this experimental study was to evaluate the epididymal necrospermiasperm death, after chronic SCI in rat. Materials and Meth...

We studied fluorescence resonance energy transfer between donors and acceptors bound to double-helical DNA. The donor Hoechst 33258 binds to the minor groove of DNA and the acceptor propidium iodide (PI) is an intercalator. The time-resolved donor decays were measured in the frequency domain. The donor decays were consistent with a random 1-dimensional distribution of acceptors. The decays were...

target of radiation action. The greatest toxicity is Purpose : The incubation of the DNA minor-groove binder observed when the I atom is incorporated within [I]iodoHoechst 33342 (IH) with plasmid DNA leads to the the DNA helix (i.e. 5-[I]iodo-2 3⁄4 -deoxyuridine, production of one double-strand break (dsb) per decay, both in IdUrd (Kassis et al. 1988)) , while its dislocation by the presence an...

cladribine, an analogue of deoxyadenosine, is highly toxic for both non-dividing and proliferating cells and has shown activity in the treatment of several malignancies. therefore, the aim of the present study is to investigate the cytotoxicity effect of cladribine (2-cda) on the breast cancer cell line, mcf-7 (estrogen receptor positive, er+). mtt assay, annexin v-fluorescein/pi and hoechst 33...

A series of flavonoid derivatives bearing trimethoxyphenyl and benzimidazole were designed, synthesized evaluated as potential anti-tumor agents. Compound 5g showed remarkable inhibitory activity against SGC-7901, HGC-27 MCF-7 (IC50 values 20.5 ± 5.93, 3.3 2.53 16.6 0.75 ?M, respectively), had great selectivity to cancer cells. The results Hoechst 33258 staining Annexin V-FITC/PI dual confirmed...

Bisbenzimidazoles have a broad spectrum of potential applications: radioprotectors, drug delivery vectors, antiviral agents, etc. At the same time, they seem to be promising fluorescent probes for radiation measurements. Therefore, in present work, response X-ray irradiation Hoechst 33258, one most widely known representatives bisbenzimidazole family, was studied first time. Irradiation dye per...

INTRODUCTION affords their differentiation from other peripheral blood cells . Previous cytological identification of It is often of interest to identify those cells in a replicating cells has employed autoradiography mixed population which are synthesizing DNA . (3-6) . These cells can now be identified by fluoresFor example, division of thymus-dependent lymcence microscopy (7, 8), due to the ...

The effect of Hoechst 33258 binding on the geometry of a DNA duplex (plasmid pBR322) has been examined using topoisomerase II relaxation followed by gel electrophoresis. Of this drug-DNA system, fluorescence, optical absorption, and calorimetric measurements were also made at various drug and DNA concentrations and in the same buffer as that for the topoisomerase reaction. It has been confirmed...