A set of 16 expressed sequence tag (EST)-derived simple sequence repeat (SSR) and 15 EST-derived single nucleotide polymorphism (SNP) markers together with 4 amplified fragment length polymorphism (AFLP) primer combinations were analyzed on 43 wild (Hordeum vulgare ssp. spontaneum – HS), 35 cultivated (H. vulgare ssp. vulgare – HV) and 12 elite (H. vulgare ssp. vulgare – from EU) barley lines. ...

Stomata of barley (Hordeum vulgare L.) and wheat (Triticum aestivum L.) leaves failed to open in the light and close in the dark or respond to changes in the CO(2) concentration of the atmosphere in either light or dark when the leaves were in an O(2)-free atmosphere. In contrast, the expected responses to environmental changes were found in atmospheres containing 1.5% O(2). It appears that O(2...

A new type of chlorophyll catabolite was isolated from extracts of de-greened primary leaves of barley (Hordeum vulgare cv. Lambic). Its constitution was elucidated by one-dimensional and two-dimensional [(1)H,(13)C]-homo- and heteronuclear NMR spectroscopic techniques and by high resolution mass spectroscopy. The isolated catabolite, a water-soluble, colorless, and nonfluorescent linear tetrap...

Increases in two extracellular peroxidases were observed following inoculation of barley (Hordeum vulgare L.) with the powdery mildew pathogen (Erysiphe graminis DC.: Fr. f. sp. hordei Em. Marchal). The more prominent isozyme, P8.5, was purified from intercellular wash fluids by acetone precipitation, ion-exchange chromatography, isoelectric focusing, and gel filtration. Purified P8.5 is a heme...

BACKGROUND AND AIMS Barley (Hordeum vulgare ssp. vulgare) cultivation started between 9500 and 8400 years ago, and was a major part of ancient agriculture in the Near East. The brittle rachis is a critical trait in the domestication process. METHODS A DNA sequence closely linked to the brittle rachis complex was amplified and resequenced in a collection of cultivated barleys, wild barleys (H....

Zestaw 478 linii jęczmienia (Hordeum vulgare L.) był badany pod kątem odporności na mączniaka prawdziwego. Badane linie wyprowadzono z odmian miejscowych jarego pochodzących głównie Basenu Morza Śródziemnego. W badaniach wykorzystano trzy izolaty Blumeria graminis o znanym profilu wirulencji. Ocenę wykonano siewkach w warunkach kontrolowanych. Badanie pozwoliło wyselekcjonować 111 odpornych jęc...

Intensive breeding has led to a narrowing in the genetic base of our major crops. In wheat, access to the extensive gene pool residing in its many and varied relatives (some cultivated, others wild) is hampered by the block on recombination imposed by the Ph1 (Pairing homoeologous 1) gene. Here, the ph1b mutant has been exploited to induced allosyndesis between wheat chromosomes and those of bo...

The effects of four environmental stresses of heat, drought, wounding and cell culture, on the transcriptional reactivation of seven long terminal repeat retrotransposons (LTRs) of barley (Hordeum vulgare) genome, were investigated. These LTRs included two Copia-type (Bare and Maximus); and five Gypsy-type (Erika, Jeli, Sabrina, Sukkula1 and Sukkula3) LTRs. RT-qPCR analyses revealed that Erika1...