BACKGROUND Chronic stress affects the course of inflammatory bowel disease and experimental colitis, and may also initiate intestinal inflammation in rats. AIM To investigate the effects of stress on the M cell containing follicle associated epithelium, specialised in antigen uptake. SUBJECTS AND METHODS Wistar rats were submitted to acute water avoidance stress for one hour or chronic wate...

Biocytin is a biotin-lysine complex of low molecular weight containing about 65% biotin, which retains a high affinity for avidin. Since the latter molecule has been conjugated to several histochemical markers, the use of biocytin as an intracellular marker was investigated. Electrodes were filled with a solution of 4-6% biocytin dissolved in 0.5 M KCl and 0.05 M Tris buffer, pH 7-7.6. Neurons ...

A collagen mafrix (COL)gel was .used to fill a 3 mm gap resulting from transection of rat sdatic nerve. The nerve was initially stabilized with a split polyethylene tubing in order to remove elastic tension at the nerve repair site. The contralateral sdatic nerve was identically prepared but the gap was left empty. Animal survival time was '9 to 12 weeks. One day prior to killing. somatosensory...

Rapid and accurate detection of phenolic wastewater from industries has created global concern. Herein, core–shell magnetic cellulose nanocrystal supported MOF (Fe3O4/CNC@ZIF-8) with robust peroxidase-like activity was synthesized tannic acid as modifier bridge. The peroxidase-mimic catalytic as-prepared Fe3O4/CNC@ZIF-8 further investigated using o-phenylenediamine (OPD) peroxidase substrates i...

The crystal structure of the major peroxidase of barley grain (BP 1) has been solved by molecular replacement and phase combination and refined to an R-factor of 19.2% for all data between 38 and 1.9 A. The refined model includes amino acid residues 1-309, one calcium ion, one sodium ion, iron-protoporphyrin IX, and 146 solvent molecules. BP 1 has the apparently unique property of being unable ...

Systematic studies on phenol derivatives facilitates an explanation of the enhancement or inhibition of the luminol-H2O2-horseradish peroxidase system chemiluminescence. Factors that govern the enhancement are the one-electron reduction potentials of the phenoxy radicals (PhO./PhOH) vs. luminol radicals (L./LH-) and the reaction rates of the phenol derivatives with the compounds of horseradish ...

New chromogens expand the colour palette for horseradish peroxidase chromogens used in immunohistochemistry. Tissue staining of cytokeratin with three new cyanine-based chromogens is described. Their use as fluorescent reporters is demonstrated.

Two engineered DNA nanostructures consisting of a nucleic acid functional hairpin and a DNA "tweezers" assembly act as pH-switchable devices for the "ON-OFF" activation/deactivation of the horseradish-peroxidase-mimicking DNAzyme.

A diverse collection of actinomycete strains were screened for production of extracellular peroxidase activity by adapting a chemiluminescence analysis system developed for horseradish peroxidase-based enzyme-linked immunosorbent assay. Extracellular peroxidase activity was found to be common but quantitatively variable, and this rapid and sensitive screening system permitted identification of ...

Clozapine is associated with a 0.8% incidence of agranulocytosis. Bioactivation to an unstable protein-reactive metabolite, identified as a nitrenium intermediate, has been implicated in the toxicity. In this study, we investigated whether the reactive metabolite is cytotoxic toward polymorphonuclear leukocytes and mononuclear leukocytes using horseradish peroxidase and H2O2 to generate the met...