High-performance liquid chromatography (HPLC) is an essential tool for the purification and characterization of biomacromolecules. This unit presents a thorough discussion of the eight types of HPLC currently used, highlighting equipment and start-up procedures, recommendations for running each type of experiment, and theoretical considerations for the separation of peptides and proteins. This ...

A high-performance liquid chromatographic (HPLC) method with UV and DAD detection for the quantitative determination of linezolid in human serum was developed in present work. Chromatography was carried out by reversed-phase technique on a RP-18 column with a mobile phase composed of 50 mM phosphate buffer and acetonitrile (76 : 26, v/v), adjusted to pH 3.5 with orthophosphoric acid. Serum samp...

BACKGROUND The potent immunosuppressant mycophenolic acid (MPA) is metabolized to an inactive glucuronide (MPAG). The extent of metabolism varies among individuals, and the MPAG formed can be hydrolyzed to MPA and can displace MPA from serum albumin, creating a potential need to monitor both MPA and MPAG. METHODS After addition of the carboxybutoxy ether of MPA (MPAC) as internal standard, MP...

wheat flour is a commodity with a high risk of aflatoxins (afs) contamination. during the bread making there are many processes that can affect the afs stability. the effect of bread making process using different yeast types on afs levels was investigated. for this purpose, standards of afs including b and gwere added to flour and then bread loaves were prepared. three types of commercially av...

raspberry waste, obtained from two cultivars, meeker (erm) and willamette (erw) was subjected to evaluation antioxidants and antitumour activities as a potential source of phenolics. some phenolic acids and flavonoids were identified and quantified by hplc. antioxidant activity was tested by measuring ability to scavenge dpph• and •oh by esr spectroscopy. ic50dpph• varied from 0.67 for erm to 0...

in this study, a high-performance liquid chromatographic method (hplc) and uv spectrophotometric method were developed, validated and applied for the determination of theophylline in biological fluids. liquid- liquid extraction is performed for isolation of the drug and elimination of plasma and saliva interferences. urine samples were applied without any extraction. the chromatographic separat...

gamma tocopherol content of 7 iranian sesame seeds (sesamum indicum l.) was determined by high performance liquid chromatography using c8 column and methanol, water and butanol as mobile phase. the gamma-tocopherol content varied from 563 to 1095 mg/kg in oil and 293 to 569 mg/kg in sesame seed and was in broad agreement with the codex range. a large variation was found in the concentration of ...

clomipramine is a tricyclic antidepressant. different methods for determination of clomipramine hydrochloride in plasma have been described. most of these procedures favor the use of acidic back-extraction in extraction procedure and hplc as the analytical technique. in this study, the clomipramine extraction procedure was modified and a direct injection to the column was performed to shorten t...