HDL-cholesterol (HDL-C) values measured by precipitation (sodium phosphotungstate-MgCl2) and direct methods were compared with those obtained by HPLC with a new column (TSKgel Lipopropak) and an eluent (TSKeluent LP-1). The HDL-C values determined by the precipitation method were significantly (P <0.001) lower than those by the HPLC method, whereas the HDL-C values by the direct method were sli...

HPLC analysis is important for determination of total level on DNA methylation in plants. It can be used to help characterise epigenetic changes during growth, development and stress. HPLC methods have been optimised for mammalian and microbial DNA, but not for plants. This article examines several important factors in the HPLC analysis of plant DNA methylation including extraction and purifica...

A phytochemical investigation of a southern Australian marine brown alga, Sargassum paradoxum, resulted in the isolation and identification of four new (5, 9, 10, and 15) and nine previously reported (1, 2, 6-8, and 11-14) bioactive meroditerpenoids. HPLC-NMR and HPLC-MS were central to the identification of a new unstable compound, sargahydroquinal (9), and pivotal in the deconvolution of eigh...

High-performance liquid chromatography (HPLC) with UV, circular dichroism (CD) and intrinsic fluorescence detection was applied to monitor conformational properties of recombinant human interferon alpha2b when performing size exclusion chromatography (SEC) and reversed-phase HPLC (RP-HPLC). In this way native conditions during SEC and structural changes of the protein during RP-HPLC were demons...

OBJECTIVES To develop and optimize quantitative HPLC method using 2,3-naphthalenedicarboxaldehyde (NDA) after simple and efficient solid phase extraction to determine the histamine in a biopharmaceutical (Histobulin™). METHODS The HPLC method was established using NDA-induced Histobulin and compared with the recently reported HPLC method using o-phthaldehyde (OPA). The validated NDA-applied H...

BACKGROUND Information on interlaboratory variation and especially on methodological differences for plasma total homocysteine is lacking. METHODS We studied 14 laboratories that used eight different method types: HPLC with electrochemical detection (HPLC-ED); HPLC with fluorescence detection (HPLC-FD) further subdivided by type of reducing/derivatizing agent; gas chromatography/mass spectrom...

aflatoxin b1 (afb1) is a secondary metabolite produced by some aspergillus species, which haveharmful impacts on human health such as carcinogenicity, teratogenicity, acute and chronic toxicity.in this study, the occurrence of afb1 in pistachio nuts during various preparing processes wasinvestigated in two pistachio farms from qazvin province, iran, from september to november 2012.high performa...

the objective of the present study was to evaluate the pharmacokinetic parameters and bioavailability of a selective histamine (h1)-receptor antagonist, cetirizine hydrochloride (ctz), following administration of a single oral dose of the drug. the properties of a test compound were compared with those of a reference product in a randomized cross-over study in 12 volunteers. blood samples were ...

a sensitive, accurate and rapid reverse phase hplc method was described to quantitate levels of acyclovir in human plasma. the drug, internal standard (metronidazole) and phosphate buffer (0.05 m) were added to serum samples and vortexed for 30 sec. a mixture of isopropyl alcohol:dichloromethane (60:40) was then added and vortexed for 3 min. samples were centrifuged and the supernatant layer wa...