High resolution melting (HRM) is a novel, closed-tube, post-PCR technique allowing genomic researchers to easily analyze genetic variations in PCR amplicons. This technical note describes general steps of setting up HRM-based PCR assays, with a special focus on ways how to optimize procedures for gene scanning experiments. The novel High Resolution Melting (HRM) technique enables researchers to...

rouk@ut . Ruël). Summary The success of information technology (IT) projects is highly dependent upon the end-users’ behaviour. Whether end-users are able and willing to work with newly introduced software applications is fundamental. Hence, a key issue is supporting targeted employees of newly introduced software applications in their proper utilisation. HRM practices have the potential to pro...

Many hierarchical reliable multicast (HRM) protocols deploy repair proxies that perform local recovery and feedback consolidation. Placement of proxies is a key design issue in HRM network. The objective of Greedy-MRS algorithm is to place the HRM proxy in a single iteration, but it requires communication with routers frequently to find the efficient proxy. The objective of preprocessing step i...

High resolution melt (HRM) is gaining considerable popularity as a simple and robust method for genotyping sequence variants. However, accurate genotyping of an unknown sample for which a large number of possible variants may exist will require an automated HRM curve identification method capable of comparing unknowns against a large cohort of known sequence variants. Herein, we describe a new ...

Esophageal impedance monitoring and high-resolution manometry (HRM) are useful tools in the diagnostic work-up of patients with upper gastrointestinal complaints. Impedance monitoring increases the diagnostic yield for gastroesophageal reflux disease in adults and children and has become the gold standard in the diagnostic work-up of reflux symptoms. Its role in the work-up for belching disorde...

A number of studies have noted the influence of national differences on HRM strategic choice and practices of multinational companies (MNC's). These have mostly concerned the influence of the characteristic s of a host country on management practice in the subsidiaries, that is an ethnocentric strategy or the process of forward diffusion. In particular they have focussed on the way in which the...

Background: The High Resolution Melting (HRM) method is a new scanning method for detecting unknown changes in DNA and its advantages have persuaded researchers to recruit it as a screening method. Objectives: Here, we developed a HRM method to screen R188H SNP (rs3218536) of XRCC2 and compared the results with a well known PCR-RFLP technique. Materials and Methods: Genomic ...

BACKGROUND In recent years human resource management (HRM) has been seen as an important factor in the successful realisation of organisational change programmes. The UK NHS is undergoing substantial organisational change and there is a need to establish which human resource (HR) initiatives may be most effective. OBJECTIVES To assess the results from a wide-ranging series of systematic revie...

Evidence-based movements have emerged recently in various disciplines. They are motivated to improve practice by making researchinformed decisions. However, the concept of “evidence-based” is easily misconstrued, risking a lost opportunity to bridge the gap between practitioners, academics and other stakeholders. As an inter-disciplinary field, e-HRM is likely to exhibit the research-practice g...

In this paper an attempt is made to gain further insight into the environment–strategy– performance linkages. A framework is developed to relate managers’ perceptions of their market environment and competitive strategies to the (advanced) production technologies and human resource management (HRM) policies adopted by their firms. Data from 12 Dutch and 8 British companies in the chemical and f...