BACKGROUND/OBJECTIVES Inonotus obliquus (I. obliquus, Chaga mushroom) has long been used as a folk medicine to treat cancer. In the present study, we examined whether or not ethanol extract of I. obliquus (EEIO) inhibits cell cycle progression in HT-29 human colon cancer cells, in addition to its mechanism of action. MATERIALS/METHODS To examine the effects of Inonotus obliquus on the cell cy...

Objective: The aim of this study was to observe the apoptotic/cytotoxic effects exogenous SPARC on colon cancer cell line HT-29, then investigate function stabilin-1 and integrin αvβ3, which are possible receptors for in cells determine quantitation their receptor numbers.
 Methods: Appropriate doses it’s inhibitor, cilengitide added HT-29 were determined by xCELLigence Real-Time Cell Anal...

Luteolin is 3',4',5,7-tetrahydroxyflavone found in celery, green pepper, and perilla leaf that inhibits tumorigenesis in animal models. We examined luteolin-mediated regulation of cell cycle progression and apoptosis in the HT-29 human colon cancer cell line. Luteolin decreased DNA synthesis and viable HT-29 cell numbers in a concentration-dependent manner. It inhibited cyclin-dependent kinase ...

The second generation peptide CIGB-552 has a pro-apoptotic effect on H460 non-small cell lung cancer cells and displays a potent cytotoxic effect in HT-29 colon adenocarcinoma cells though its action mechanism is ill defined. Here, we present the first proteomic study of peptide effect in HT-29 cells using subcellular fractionation, protein and peptide fractionation by DF-PAGE and LC-MS/MS pept...

Background and objective: Previous studies have demonstrated the anti-cancer effects of silymarin (SLM). However, the low bioavailability of SLM has restricted its use. This study investigated the toxic effect of nanostructured SLM encapsulated in micelles (Nano-SLM) on the growth of the HT-29 human colon cancer cell line. Materials and methods: HT-29 cells were treated with 25 μM/mL of SLM or ...

Uridine-cytidine kinase 2 is an enzyme that is overexpressed in abnormal cell growth and its implication is considered a hallmark of cancer. Due to the selective expression of UCK2 in cancer cells, a selective inhibition of this key enzyme necessitates the discovery of its potential inhibitors for cancer chemotherapy. The present study was carried out to demonstrate the potentials of natural ph...

Objectives: The present study is to investigate the effect of quercetin on cisplatin-induced growth inhibition, apoptosis and nuclear factor-kappa B (NF-κB) expression in colon cancer HT-29 cells. Methods: HT-29 cells were treated with quercetin, cisplatin or the combination of both. MTT assay was applied to examine cell viability. Transmission electron microscopy and laser scanning confocal mi...

Targeted therapy treatment in Colorectal Cancer (CRC) works specifically on a protein or target cell cancer. It plays role the survival of cancer cells. involves several monoclonal antibodies, one which is cetuximab targets Epidermal Growth Factor Receptor (EGFR). However, there are cases resistance caused by activities Tissue Inhibitor Metalloproteinase-1 (TIMP-1). TIMP-1 can activate downstre...

recent reports have indicated different effects of immunostimulatory sequences containing cpg-oligodeoxynucleotides (odn) on various immune cells. however, the exact role of cpg-odn in the human gut is unclear. in the present study, we assessed potential effects of cpg-odn on non lymphoid cell (intestinal epithelial cell line ht-29) on a dose-response and time-course basis. intestinal epithelia...

Nonsteroidal anti-inflammatory drugs (NSAIDs) decrease the incidence of and mortality from colon cancer. We observed that NSAIDs inhibit the proliferation rate, alter the cell cycle distribution, and induce apoptosis in colon cancer cell lines. We evaluated whether the inhibition by NSAIDs of prostaglandin (PG) synthesis is required for their effects on colon cancer cells by studying two human ...