The current study aimed to examine the percentage changes of viability sperm and activity enzymes LDH, ALP, GOT / AST, GPT ALT in plasma Lacaune rams, before after cryopreservation. For this purpose, five rams were examined, two ejaculates obtained from each ram. Ejaculates are collected by method artificial vagina, during insemination campaign. All diluted with a 6AG extender frozen Cassou?s s...

Evaluation of the quality of cryopreserved bull spermatozoa is reviewed. The methods for the assessment of sperm quality have markedly improved over the last decades – starting with the assessment of morphological shapes and subjective motility analysis towards the more sophisticated analysis of the molecular changes in chromatin, membranes and catabolic activities of the sperm cell itself. Fun...

Cryopreservation of sperm has many applications in agriculture, biotechnology, and clinical medicine. The spermatozoon is a very specialized cell that loses the ability of biosynthesis, repair, growth and cell division during the final phase of spermatogenesis. Cryopreservation of sperm generally requires a reduction or arrest of the metabolism of cells, thereby prolonging their life. Semen sam...

AIM To study whether additional measurements of motility characteristics of spermatozoa by computer assisted semen analysis (CASA) were more sensitive indicators of reduced semen quality than estimates of percentages of motile, rapid or progressive cells. METHODS Intermittent scrotal insulation was applied to 6 rams for 16 h per day for 21 days or to 2 of these for 12 h per day for 28 days in...

Strategies to preserve fertility in dogs and cats as cryopreservation of epididymal spermatozoa, oocytes and ovarian tissue will be discussed in this review. Recent results indicate that significant progress have been achieved, but optimal freezing protocols have not been defined yet. The extreme sensitivity of sperm membranes and of oocyte and ovarian tissue structure deserves further investig...

BACKGROUND Sperm parameters and motion kinetics are affected by cryopreservation. OBJECTIVE The main purpose of the current study was to determine the effect of different concentrations of Trolox as an antioxidant to freezing-thawing procedure on human sperm kinematic parameter. MATERIALS AND METHODS Semen was collected from 20 normal donors and divided into five aliquots prior to cryoprese...

background: cryopreservation of semen requires optimized conditions to minimize the harmful effects of various stresses. the main approach for protection of sperm against stress is based on the use of antioxidants and cryoprotectants, which are described as defensive methods. recently, the application of controlled mild stressors has been described for activation of a temporary response in oocy...

The aim of this study was to compare the effect of different packaging systems on some parameters of cryopreserved canine spermatozoa. The experimental material consisted of the sperm-rich fractions of ejaculates collected from four Beagle dogs. Semen samples for cryopreservation were stored in 0.25 ml plastic straws and two aluminum tubes with a total volume of 5.0 ml. Semen was frozen in stat...

This study aimed to analyze the effect of Alpinia purpurata K. Schum rhizome extract (APRE) supplementation into tris-egg yolk extender on sperm quality Bali bull after freezing. used experimental method with a completely randomized design (CRD), consisting five treatments, denoted as P0 (semen diluted using 100% Tris-Egg Yolk (TEY)), P1 98% TEY and 2% APRE), P2 96% + 4% P3 94% 6% P4 92% 8% APR...

A continuous decline in the number and range of capercaillie (Tetrao urogallus L.) in many European countries can be observed, mostly due to habitat destruction by human activity, unecological forestry management, and increased density of natural predators. Ex situ in vitro gene banks provide a unique opportunity to preserve the genetic material for future generations. Simple and effective cryo...