The aim of the present study was to evaluate the protective effect of L-glutamine (L-Gln) against cryopreservation injuries on boar sperm. In Experiment 1, L-Gln from 20 to 80 mM was evaluated as a supplement for a standard freezing extender (egg yolk - EY - 20%, and glycerol 3%). No significant improvement (P>0.05) was obtained for any post-thaw sperm parameter assessed (objective sperm motili...

introduction: to evaluate the quick cryopreservation technique on parameters of motility, morphology, and vitality of spermatozoa from normozoospermic ejaculates. materials and methods: in this prospective study, a total of 37 normal ejaculates were evaluated according to who criteria, then each sample was divided into two aliquets and sample was cryopreserved with quick freezing method. sperm ...

OBJECTIVE Comparison of human sperm motility, morphology, and sperm membrane integrity using two cryoprotective media, Test-yolk buffer with glycerol and glycerol alone. METHODS Semen samples from 10 healthy donors were divided and frozen with either glycerol or a combination of glycerol and TEST-yolk buffer. Semen characteristics were evaluated before freezing and after thawing. Motility was...

A major cause of male infertility, which includes 50 causes of infertility, is oxidative stress.  The aerobic respiration in the sperm cell is associated with the production of reactive oxygen species (ROS). Some reactions such as sperm capacitation and acrosome reactions require moderate levels of ROS. However, when the amount of ROS production surpasses the natural antioxidant defense of the ...

Cryopreservation of few spermatozoa is still a major challenge for male fertility preservation. This study reports use a new micro-straw (LSL straw) for freezing few spermatozoa for intracytoplasmic sperm injection (ICSI). Semen samples from 22 fertile donors were collected, and each semen sample was diluted and mixed with cryoprotectant in a ratio of 1:1, and then frozen using three different ...

Changes in sperm structure and function occur during the processing of semen. The present study was designed to investigate the effect on buck sperm during different stages of semen preparation including dilution, cooling, equilibration and freeze-thawing. Semen ejaculates from three mature bucks (replicates = 5) were diluted with tris-citric acid egg yolk glycerol extender at 37 ºC, cooled to ...

BACKGROUND Intracytoplasmic Sperm Injection (ICSI) is an Assisted Reproduction Technique (ART) which offers the chance to conceive to patients presenting very low sperm counts (cryptozoospermia/severe oligozoospermia). Sperm freezing before the oocyte pick-up, can prevent from a lack of spermatozoa on the day of the ICSI. It can avoid the cancellation of the ICSI or the use of TESE (Testicular ...

Problem statement: Boar sperm cells are sensitive to the freezing process, which compromises viability of frozen-thawed sperm. In a constant search for minimizing or suppressing sperm cell damage caused by the temperature and osmolarity changes during the freezing process, crioprotective and antioxidant substances have been added to the freezing media, such as coconut water, in order to increas...

It is thought that differences in conception rate between feline epididymal sperm and ejaculate sperm occur because, unlike ejaculated sperm, caudal epididymal sperm have not been sensitized with seminal plasma (SP). In this study, we investigated whether collection of feline epididymal sperm with SP influences sperm qualities after freezing-thawing. Sperm were sensitized with SP for 10 min at ...