OBJECTIVE To assess the role claudin-5, an endothelial cell (EC) tight junction protein, plays in establishing basal permeability levels in humans by comparing claudin-5 expression levels in situ and analyzing junctional organization and function in 2 widely used models of cultured ECs, namely human dermal microvascular (HDM)ECs and human umbilical vein (HUV)ECs. METHODS AND RESULTS By immuno...

For this lecture, consider a graph G = (V,E), with n = |V | and m = |E|. Let du denote the degree of vertex u. Recall some of the quantities we were interested in from last time: Definition 28.1.1 The transition matrix is a matrix P such that Puv denotes the probability of moving from u to v: Puv = Pr[random walk moves from u to v given it is at u]. Definition 28.1.2 Stationary Distribution for...

SUMMARY OBJECTIVES The main objective of this study was to describe the clinical characteristics of patients diagnosed with penile fracture in the Hospital Universitario del Valle (Cali, Colombia). METHODS A descriptive study, reviewing all the medical records of patients diagnosed with penile fracture from January 2001 to December 2008 at Hospital Universitario del Valle (.HUV).in Cali. It t...

BACKGROUND Death receptors (DRs) play an important role in renal pathology. We have shown that DR3 is inducibly expressed on renal tubular epithelial cells in the setting of inflammatory injuries. In this study we investigate the expression of DR3 in renal endothelial cells and their response to TL1A, the only known ligand of DR3. METHODS We did RT-PCR, flow cytometry and subcellular immunobl...

An association between platelets, angiogenesis, and cancer has long been recognized, but the mechanisms linking them remains unclear. Platelets regulate new blood vessel growth through numerous stimulators and inhibitors of angiogenesis by several pathways, including differential exocytosis of angiogenesis regulators. Herein, we investigated the differential release of angiogenesis stimulators ...

To appraise the health economic evidence for adjuvant chemotherapy (AC) strategies in stage II and III colorectal cancer (CRC) to identify limitations of available that might inform further research. A systematic review published evaluations was undertaken. Four databases were searched full-text publications English screened inclusion. The Consolidated Health Economic Evaluation Reporting Stand...

ERG (Ets-related gene) is an ETS transcription factor that has recently been shown to regulate a number of endothelial cell (EC)-restricted genes including VE-cadherin, von Willebrand factor, endoglin, and intercellular adhesion molecule-2. Our preliminary data demonstrate that unlike other ETS factors, ERG exhibits a highly EC-restricted pattern of expression in cultured primary cells and seve...

Heat shock proteins (HSPs) are a set of highly conserved proteins that can serve as intestinal gate keepers in gut homeostasis. Here, effects of a probiotic, Lactobacillus rhamnosus GG (LGG), and two novel porcine isolates, Lactobacillus johnsonii strain P47-HY and Lactobacillus reuteri strain P43-HUV, on cytoprotective HSP expression and gut barrier function, were investigated in a porcine IPE...

OBJECTIVE Resveratrol, a natural phytoalexin compound, is present in grapes and wine, and it can produce vasorelaxation. However, little is known of its mechanisms of action on ionic currents in endothelial cells. METHODS The effect of resveratrol on Ca(2+)-activated K+ currents in an endothelial cell line (HUV-EC-C) originally derived from human umbilical vein was investigated with the aid o...