Solutions made by homogenization of vitreous humor exhibit anomalous viscosity at low shear rates due to interaction between hyaluronic acid and a hydroxyproline-containing material similar to collagen. The latter can be removed by filtration or centrifugation to give a solution whose viscosity is due to hyaluronic acid. On concentration the viscosity of the latter remains independent of shear ...

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background: various approaches have been used in the attempts to improve the quality of frozen–thawed mouse sperms. according to literatures, it seems that hyaluronic acid (ha) has an important role on the permeability and motility of sperms and their interaction with gametes. objective: for evaluation of ha supplementation on sperm characteristics and fertilization capability, we investigated ...

The hyaluronic acid-binding region was prepared by trypsin digestion of chondroitin sulfate proteoglycan aggregate from the Swarm rat chondrosarcoma, and biotinylated in the presence of hyaluronic acid and link protein. After isolation by gel filtration and HPLC in 4 M guanidine HCl, the biotinylated hyaluronic acid-binding region was used, in conjunction with avidin-peroxidase, as a specific p...

Previous reports from this laboratory demonstrated the synthesis of hyaluronic acid by a cell-free preparation obtained from a strain of Group A streptococcus. The enzymic activity which sedimented at 105,000 x g, but not at 10,000 x g, required the addition of uridine diphospho-N-acetylglucosamine, uridine diphosphoglucuronic acid, and Mg++ for the formation of hyaluronic acid (l-3). Since hya...

The main object of this paper is to report measurements made on a small series of human synovial fluids and on a few sheep and ox fluids. These measurements were made in order to test the two methods suggested by Ogston & Stanier (1953 a) for detecting differences of the specific properties of the hyaluronic acid as it occurs in synovial fluids. These methods, both of which are based on viscosi...

Introduction: Hearing loss is fairly a common disorder which is usually treated with corticosteroids via systemic administration and/or intra-tympanic injection. This study aimed to compare the effectiveness of intra-tympanic injections of dexamethasone with its combination with hyaluronic acid in patients with sudden sensorineural hearing loss.   Materials and Methods: In this clinical trial, ...

Section on Tissue Biophysics and Biomimetics, NICHD, National Institutes of Health, 13 South Drive, Bethesda, MD 20892, USA E-mail: [email protected] Summary: Aggrecan is a charged biopolymer whose complexes with hyaluronic acid provide the compressive resistance of cartilage. In cartilage extracellular matrix aggrecan-hyaluronic acid complexes are enmeshed within a network of collagen fibr...

Total tissue content and molecular mass of hyaluronic acid was determined in papain digests of human articular cartilage using a sensitive radiosorbent assay [Laurent & Tengblad (1980) Anal. Biochem. 109, 386-394]. 1) Hyaluronic acid content increased from 0.5 microgram/mg wet wt. to 2.5 micrograms/mg wet wt. between the ages of 2.5 years and 86 years. 2) Hyaluronic acid chain size decreased fr...

BACKGROUND Although the safety profile of hyaluronic acid fillers is favorable, adverse reactions can occur. Clinicians and patients can benefit from ongoing guidance on adverse reactions to hyaluronic acid fillers and their management. METHODS A multinational, multidisciplinary group of experts in cosmetic medicine convened the Global Aesthetics Consensus Group to review the properties and c...