Background Hybridoma technology was established in the 2nd half of the 20th century and in the view of current protein production it might seem old-fashioned. Despite, it is commonly used to produce monoclonal antibodies (mAbs) for R & D, clinical diagnostics or medical applications and the demand for mAbs produced by hybridomas is still high. However, compared to CHO, only a few serum-free hyb...

Hybridoma cells which secrete colorectal carcinoma-specific antibodies have been produced and used to study the antigenic structure of these tumor cells. Nineteen antibodies have been studied in detail, and 15 of these are colorectal carcinoma specific. Only two antibodies reactive with carcinoembryonic antigen (CEA) have been discovered and five other antibodies that react with distinct epitop...

Three major groups of antibodies have been identified during the analysis of the clonal response to phosphoeholine (PC). t The predominant one shows multiple molecular features of the PC-binding myeloma protein (PC-BMP) TEPC 15 (1-3), and two others possess idiotypes (Id) and L-chain characteristics of two other PCBMP, M511 (2, 4) and M603 (5). All three Id groups are conserved in inbred mice (...

Many hybridoma and recombinant myeloma cell lines have been successfully adapted to growth in protein-free media. Compared with serum-supplemented media, use of protein-free media promotes superior cell growth and protein expression and facilitates downstream purification of the expressed product. Owing to its sterol auxotrophy, the NS0 myeloma is normally grown in either a serum-supplemented m...

Immunoglobulin production stimulating activity of polyamines was investigated. Spermidine, thermine and triethylenetetraamine (TETA) stimulated IgM production of human-human hybridoma, HB4C5 cells under serum-free condition. IgM production of HB4C5 cells was accelerated 5.9-, 5.3-, and 3.7-fold by spermidine at 4.5 mM, thermine at 2 mM and TETA at 2.5 mM, respectively. However, putrescine did n...

It has been well established that monoclonal antibodies, since their initial description', play an important role on biological research . This technique has also been applied to studies on plant virus2·6> and this aspect was reviewed >. The basic characteristics of monoclonal antibodies are that (1) the antibody specifically reactive with one epitope can be obtained, (2) a hybridoma clone, onc...

Murine monoclonal hybridoma antibodies were generated efficiently by using a human leukemia antigen preparation which was isolated from the cell membranes of MOLT-4 (a T-leukemia cell line) by means of a novel system. The generated monoclonal hybridoma antibodies were screened and characterized by a radioimmunoassay using a variety of cell specimens as targets. The results showed that the antig...

Abstract IgA nephropathy (IgAN) is the most common type of autoimmune glomerulonephritis that frequently progresses to end-stage renal disease. However, molecular pathogenesis underlying IgAN remains largely unknown. This study investigated role galectin-3 (Gal-3), a galactoside-binding protein in pathogenesis. Two complementary mouse models, model induced with TEPC-15 hybridoma using Gal-3 kno...

The fusion of an AKR T cell tumor line to normal B6D2F1, T cells resulted in the production of a cloned T cell hybridoma (FS6-14.13) inducible with the mitogen concanavalin A (Con A). The supernate from Con A-stimulated hybridoma cells was active both in the stimulation of an anti-sheep red blood cell response by partially T cell-depleted B cells and in the stimulation of the growth of antigen-...