Three major groups of antibodies have been identified during the analysis of the clonal response to phosphoeholine (PC). t The predominant one shows multiple molecular features of the PC-binding myeloma protein (PC-BMP) TEPC 15 (1-3), and two others possess idiotypes (Id) and L-chain characteristics of two other PCBMP, M511 (2, 4) and M603 (5). All three Id groups are conserved in inbred mice (...

Hybrid hybridomas (also termed quadromas or tetradomas) are man-made cell lines that secrete bispecific antibodies (bsAb) with two different specificities being able to crosslink two distinct molecules. Such antibodies do not occur in nature and have been originally developed to improve immunohistochemical staining procedures and immunoassays (Milstein and Cuello 1983; Suresh et al. 1986). Inte...

Objective(s) The main objective of this study was to investigate the status of chromosome stability in 3 human-mouse hybridoma cell lines over a period of time in various passages. Materials and Methods Metaphase spreads from 3 human-mouse cell lines (HF2X653, SPMO-4 and F3B6) that had been cultured in 4 successive passages, from 1 to 4 weeks, were prepared and analyzed. Metaphase chromosome...

inhibitor of the lipid peroxidation induced by iron salts and ascorbic acid.7) It has been revealed that mammalian cells in culture do not always Tequire transferrin, ifiTon is provided as a complex with appropriate chelators.8) To design a protein-free medium for hybridomas, we studied the effect of ferric citratc as an alternative to transferrin on thc proliferation of hybridomas. Mouse hybri...