OBJECTIVE Sinapic acid (SA, Sinapine), small naturally occurring hydroxycinnamic acid, has a GABA(A) receptor agonistic property and free radical scavenging activity. We examined potential neuroprotective effects of sinapic acid (SA) using global cerebral ischemia animal model. METHODS MTT assay was performed to determine cytotoxic effects of SA. To examine the neuroprotective effects of SA, ...

Polyphenol compounds, such as caffeic acid, are important substrates of oxidation in beverages such as wine, and more rapid and sensitive methods of analysis are being sought. The conducting polymer poly-3,4-ethylenedioxythiophene (PEDOT) provides good separation of caffeic acid from antioxidant additives in wines, and produces a clear anodic peak that can be used to quantify caffeic acid and r...

A novel method for the rapid screening of clandestine tablets for drugs by MALDI-TOF mass spectrometry is described. In this method, cetrimonium bromide (CTAB), a surfactant, is added to the conventional alpha-cyano-4-hydroxycinnamic acid (CHCA) matrix solution used in preparing the MALDI samples. This procedure allows very clean mass spectra to be collected for amphetamine, methamphetamine, 3,...

The brown marmorated stink bug (BMSB; Halyomorpha halys Stal.) has become a significant pest in Slovenia, especially apple, pear, peach, and cherry orchards. In our study, apple fruit of the cultivar ‘Red Pinova’ were evaluated for visual injury sugar, organic acid, phenolic contents. chemical composition area around puncture wound, uninjured part infested was compared to, as control, only appl...

Renal fibrosis is a crucial pathological phenomenon of chronic kidney disease (CKD) that contributes to the progressive loss renal function. Monocarboxylate transporter 1 (MCT1) plays an important role in clear cell carcinoma; however, MCT1 inhibitor alpha-cyano-4-hydroxycinnamic acid (α-CHCA) remains unclear. In this study, we evaluated α-CHCA, inhibitor, treating associated with CKD. We used ...

The effect of selected potential inhibitors of anion transport mediated by the Band 3 protein of the erythrocyte membrane was studied by the electron spin resonance (ESR) technique using Tempo-phosphate spin probe. No inhibition of Band 3-mediated transport by 1-cyano-4-hydroxycinnamic acid, inhibitor of monocarboxylate transporters (MCT) was found but inhibition by some polyphenols (curcumin >...

We report covalent attachment via a thiol ester linkage of 3,5-dimethoxy-4-hydroxycinnamic acid (sinapinic acid or SA) to cysteine-containing protein biomarkers from bacterial cell lysates of E. coli analyzed by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry when using SA as the matrix. Evidence to support this conclusion is the appearance of additional peaks in the MS sp...