Newcastle disease virus (NDV) strain F is a lentogenic vaccine strain used for primary vaccination in day-old chickens against Newcastle disease (ND) in India and Southeast Asian countries. Recombinant NDV-F virus and another recombinant NDV harboring the major capsid protein VP2 gene of a very virulent infectious bursal disease virus (IBDV); namely rNDV-F and rNDV-F/VP2, respectively, were gen...

Infectious bursal disease (also known as IBD, Gumboro Disease, Infectious Bursitis and Infectious Avian Nephrosis) is a highly contagious disease of young chickens caused by infectious bursal disease virus (IBDV), characterized by immunosuppression and mortality generally at 3 to 6 weeks of age. The disease was first discovered in the USA, near the town of Gumboro, Delaware in 1962. It is econo...

A rapid and sensitive dot-ELISA test was developed for the simultaneous detection of antibodies against infectious bursal disease (IBD), hydropericardium syndrome (HPS) and chicken anaemia (CAV) viruses in a single serum sample. The developed dot-ELISA was compared with the virus neutralization test (VNT). For IBD and HPS, viruses isolated and purified from field outbreak materials were used wh...

Structural proteins of the poultry pathogen, infectious bursal disease virus (IBDV), were expressed in the baculovirus/insect cell expression system. To date, several reports have indicated that animal virus structural proteins are expressed only at low yield in this system. In this article, several factors were examined to enhance yield. These include medium, dissolved oxygen level, and the ad...

Preparations of density gradient-purified infectious bursal disease virus (IBDV) were found to contain full and empty icosahedral virions, type I tubules with a diameter of about 60 nm, and type II tubules 24 to 26 nm in diameter. By immunoelectron microscopy we demonstrate that virions and both types of tubular structures specifically react with anti-IBDV serum. In infected cells intracytoplas...

This study aims to produce an effective subunit vaccine against infectious bursal disease virus (IBDV). The genes of chicken interleukin-2 (ChIL-2) and IBDV viral protein 2 (VP2) were amplified and fused by splice overlap extension-polymerase chain reaction (SOE-PCR). The fusion gene was digested by EcoR I/Kpn I and inserted into pBacPAK8 vector, resulting in recombinant transfer plasmid pBacPa...

During the period from July 2002 to June 2003, infectious bursal disease (IBD) was suspected in 101 commercial flocks of broiler chickens on the basis of clinical and post mortem findings in some districts of the Haryana state, India. Bursal samples were collected randomly from 20 flocks for the detection of infectious bursal disease virus (IBDV) by reverse transcriptionpolymerase chain reactio...