We have examined the ability of extracellular ATP to elicit intracellular Ca2+ mobilization in a broad range of human leukocytes at particular stages of hematopoietic differentiation. The average cytosolic [Ca2+] in various leukocyte populations was measured in Fura 2-loaded cell suspensions while the cytosolic [Ca2+] in individual, Indo 1-loaded leukocytes was assayed by flow cytometric method...

Background: The incidence of chronic liver disease is increasing worldwide. Common causes include alcohol, hepatitis B and C non-alcoholic fatty (NAFLD). Abnormalities in hematological parameters are common patients with cirrhosis. There paucity data geriatric population diseases.Methods: A pre-structured performa was prepared to collect the demographic that included Age, Gender, Occupation, Ar...

The Ikaros gene encodes a zinc finger transcription factor that is selectively expressed by all hematopoietic cells. Although Ikaros is required for lymphocyte differentiation, its role in the myeloid lineage is unclear. We show here that Ikaros expression is temporally regulated during neutrophil differentiation: Ikaros is primarily expressed at immature stages and significantly less so in mat...

INTRODUCTION Patients with systemic lupus erythematosus (SLE) have an abnormal population of neutrophils, called low-density granulocytes (LDGs), that express the surface markers of mature neutrophils, yet their nuclear morphology resembles an immature cell. Because a similar discrepancy in maturation status is observed in myelodysplasias, and disruption of neutrophil development is frequently ...

Qualitative tests for C-reactive protein (CRP) are available for use in dogs, and provide a rapid in-house method of detecting acute inflammation. The aim of this study was to compare results from a qualitative CRP lateral flow test (Teco CRP FASTest) to those obtained from a quantitative CRP ELISA and to traditional methods of detecting inflammation, including total leukocyte and neutrophil nu...

The purpose of this study was to describe and compare the clinical, laboratory and health care characteristics of newborns (NBs) with confirmed late onset sepsis and NBs with unconfirmed late sepsis, verify if there were any differences between the groups, and describe the germs prevalent in the studied neonatal unit. This is a descriptive study, involving 168 cases. It was observer that 33.3% ...

Abstract Background Introduction: Neutrophils and neutrophil extracellular traps (NETs) play a significant role in the pathogenesis of ulcerative colitis (UC), chronic relapsing inflammatory disease colon. Low-density neutrophils (LDNs) are recently identified subset that more prone to develop NETs produce pro-inflammatory cytokines calprotectin than normal density. Moreover, LDNs show reduced ...

Neutrophils are the most abundant immune cell in circulation. However, due to a number of technical challenges for researchers, including neutrophil's short lifespan and difficulties with preservation, they often discarded during blood processing thus ignored cohort studies. As such, contribution neutrophils disease their involvement mechanisms is less explored compared other types. Systemic lu...