non-enzymatic glycosylation of proteins is one of the key mechanisms in the pathogenesis of diabetic complications. glycation of igg is of special interest due to its possible influence on the functionality of immunoglobulins and overall immuno-competence. the aim of this study was to clarify more details of in vitro glycation of igg and to study the effect of this modification on its interatio...

Stable aggregated IgG (A-IgG) of various sizes, having high biological activity, were incubated at 4 degree C with adhering peritoneal macrophages from normal rats and the kinetics of A-IgG binding to the cell surface were studied. Equilibrium constants were high (2.8-11.7 X 10(8) M-1) and varied as a function of aggregate size. The maximum number of A-IgG bound per cell varied from 230,000 for...

background: it seems that the success of vaccination for cancer immunotherapy such as dendritic cell (dc) based cancer vaccine is hindered through a powerful network of immune system suppressive elements in which regulatory t cell is the common factor. foxp3 transcription factor is the most specific marker of regulatory t cells. in different studies, targeting an immune response against regulat...

This study identifies nonimmune binding of equine immunoglobulin by the causative organism of contagious equine metritis. Immunoglobulin binding to the bacterium was strongest for immunoglobulin G (IgG) and less for IgM; IgA was not bound. Binding of equine IgG was inhibited by human IgG, but not by IgG of domestic animals. Immunoglobulin binding by the bacterium appeared to be directed towards...

BACKGROUND The literature is scarce regarding the prevalence and clinical impact of IgG subclass deficiency in COPD. We investigated the prevalence of IgG subclass deficiencies and their association with exacerbations and hospitalizations using subjects from two COPD cohorts. METHODS We measured IgG subclass levels using immunonephelometry in serum samples from participants enrolled in two pr...

BACKGROUND Rituximab is a B cell depleting anti-CD20 monoclonal antibody. CD20 is not expressed on mature plasma cells and accordingly rituximab does not have immediate effects on immunoglobulin levels. However, after rituximab some patients develop hypogammaglobulinaemia. METHODS We performed a single centre retrospective review of 177 patients with multisystem autoimmune disease receiving r...

different classes of autoantibodies against two antigenic forms of modified low density lipoprotein (mod-ldl) were detected in 140 patients by indirect elisa method. investigated autoantibodes included total immunoglobulins (igt). immunoglobulin g (igg), and immunoglobulin m (igm) against oxidized ldl with copper ions (ox-ldl) and modified ldl with malondialdehyde (mal-ldl) (igt-o) igt against ...

By generating four IgG isotype-switch variants of the high affinity 34-3C anti-erythrocyte autoantibody, and comparing them to the IgG variants of the low affinity 4C8 anti-erythrocyte autoantibody that we have previously studied, we evaluated in this study how high affinity binding to erythrocytes influences the pathogenicity of each IgG isotype in relation to the respective contributions of F...