BACKGROUND Compared with FISH and qRT-PCR analyses, immunohistochemistry (IHC) is the preferred screening test in most pathology practices for ALK-rearrangement detection. With 100% sensitivity and 98% specificity, the VENTANA ALK (D5F3) IHC assay has been approved in the EU and some Asian countries for ALK-rearrangement detection. However, an automated Ventana IHC platform is not available in ...

Immunohistochemistry (IHC) is an ancillary method, widely used in pathologist practice, that allows to identify diagnostic and prognostic/predictive therapeutic response protein markers on tissue samples by the use of specific monoclonal antibodies chromogenic substances guarantee visualization antibody–antigene binding complex under light microscope [...]

BACKGROUND The immunohistochemical assessment of Ki67 antigen (Ki67) is the most widely practiced measurement of breast cancer cell proliferation; however, it has some disadvantages and thus the prognostic value of Ki67 in breast cancer remains controversial. Our previous studies confirmed the advantages of quantum dots-based nanotechnology for quantitative analysis of biomarkers compared with ...

BACKGROUNDS: Diseases affecting the respiratory tract of sheep and goats are one of the most important factors which limit production of these species on a world-wide basis. OBJECTIVES: The main goal of this study was to determine Adenovirus (AdV) antigen in formalin-fixed paraffin-embedded lung tissue of pneumonic goats, using immunohistochemistry (IHC) staining method. METHODS: For this purpo...

Although immunohistochemistry (IHC) is a popular imaging technique, the quantitative analysis of IHC images via computer-aided methods is an emerging field that is gaining more and more importance thanks to the new developments in digital high-throughput scanners. In this paper, we discuss the main steps of IHC and review the techniques for computer-aided chromogenic IHC analysis, including met...

Abstract Immunohistochemistry (IHC) is the use of antibodies as probes to determine localization proteins in a cell or tissue sample. In microscopy, technique has been since 1940s by pathologists and research scientists. At first glance it appears be straightforward procedure. practice, however, an IHC protocol several steps where problems can occur, resulting either false positive negative dat...

Abstract Immunohistochemistry (IHC) is the use of antibodies as probes to determine localization proteins in a cell or tissue sample. In microscopy, technique has been since 1940s by pathologists and research scientists. At first glance it appears straightforward procedure. However, practice, an IHC protocol number steps where problems can occur, resulting either false positive negative data. b...