BACKGROUND Immunohistochemistry (IHC) is a well-established, widely accepted method in both clinical and experimental parts of medical science. It allows receiving valuable information about any process in any tissue, and especially in bone. Each year the amount of data, received by IHC, grows in geometric progression. But the lack of standardization, especially on the post-analytical stage (in...

This review describes the foundations, methodology, applications, advantages, and disadvantages of immunohistochemistry used for the diagnosis of swine infectious disease. Immunohistochemistry (IHC) relies on the binding of immunoglobulins to antigens present in tissues. Numerous proteins from infectious agents (viruses, bacteria, parasites, fungi) can be detected with this technique. The antig...

OBJECTIVES To test whether academic centers (ACs) are more successful than nonacademic centers (NACs) in immunohistochemistry (IHC) external quality assessment challenges in the Canadian Immunohistochemistry Quality Control (CIQC) program. METHODS Results of 9 CIQC challenges for breast cancer marker (BM) and various non-breast cancer marker (NBM) tests were examined. Success rates were compa...

Aim: Comparative immunohistochemical study of expression α A Crystallin in non-cataract lenses and age-related cataract humans. Methodology: This was an observational cross sectional study. There are two groups this Group comprised 121 senile degenerative from diagnosed patients. B included 10 patients who underwent surgeries for enucleation due to trauma retinoblastoma. Lenses were fixed 10% B...

BRAF V600E mutation analysis has been an important companion diagnostic assay to evaluate prognosis of malignant melanoma and to guide target therapy with BRAF inhibitor. The aim of this study was to assess the sensitivity and specificity of Immunohistochemistry (IHC) using VE1 mutation specific antibody on screening for BRAF V600E mutation patients with cutaneous malignant melanoma. We detecte...

Immunohistochemistry (IHC) is an important auxiliary method for pathologists in routine diagnostic work as well as in basic and clinical research including exploration of biomarkers, as IHC allows confirmation of target molecule expressions in the context of microenvironment. Although there has been a considerable progress in automation and standardization of IHC, there are still many things to...

Cancers survivors with second tumour mass. The difficult thing in these situations is definitive diagnosis. One sentence which shapes the future of a patient-a steep see saw and palliative treatment. What saves clinician this scenario immunohistochemistry (IHC). It single handedly brightens path leads to conclusion. This case report demonstrated same breast cancer survivor.

We assessed the validity of immunoblotting, immunohistochemistry (IHC), and immunocytochemistry (ICC) to detect anti-neuronal antibodies in an attempt to establish a diagnostic approach for pediatric autoimmune encephalitis. Both IHC and ICC had higher sensitivity than immunoblotting and could differentiate between antibodies directed towards intracellular and cell surface antigens. There was a...

This study sought to evaluate a new combined gene and protein detection platform in the context of HER2 evaluation in breast and gastric carcinomas. HER2 immunohistochemistry (IHC) and dual color in situ hybridization (Dual ISH) were combined on a single slide. Results were compared with conventional HER2 IHC and fluorescence ISH. Results from the gene and protein assay were reliable and highly...

INTRODUCTION The BRAF V600E mutation is the most common genetic event occurring in papillary thyroid cancer (PTC). Recently, the possibility of using immunohistochemistry (IHC) to detect the BRAF V600E mutation has been reported. MATERIALS AND METHODS In 140 patients with classical PTC, the status of the BRAF V600E mutation was determined by IHC (using two alternative staining protocols, IHC-...