Multiplex immunofluorescence (mIF) visualizes multiple immune markers on a single tissue specimen while preserving spatial context. Compared to immunohistochemistry (IHC) which is limited single-marker staining, mIF allows for cell phenotyping within the tumor microenvironment and can provide clinically relevant prognostic information about response immunotherapy. Recently, our lab has validate...

Colorectal cancer (CRC) is a heterogeneous disease with different genetic and molecular backgrounds, leading to diverse patient prognosis treatment response. Four consensus subtypes (CMS 1–4) have recently been proposed based on transcriptome profiling. A clinically practical immunohistochemistry (IHC) CMS classifier consisting of the four markers FRMD6, ZEB1, HTR2B, CDX2 was then demonstrated....

Introduction: Lung cancer, with about 2.2 million new cases and 1.8 deaths, is the second most commonly diagnosed cancer leading cause of death in 2020. Immunohistochemistry (IHC) now used not only to diagnose classify lung tumors into subtypes, but also determine eligibility patients for different molecular-targeted therapies. 
 Objectives : Study ALK, EGFR PD-L1 mutations Pulmonary Carci...

Introduction: Breast cancer is one of the most common malignant tumors and leading causes related death amongst females worldwide. The clinical significance molecular classification carcinoma breast remains to be established. Molecular subtyping using immunohistochemistry can provide additional prognostic predictive information. Aim objectives: To identify morphological variants determine vario...

CD20 is expressed in most B-cell lymphomas and is a critical molecular target of rituximab. Some B-cell lymphomas show aberrant CD20 expression, and rituximab use in these patients is controversial. Here we show both the molecular mechanisms and the clinical significance of de novo diffuse large B-cell lymphomas (DLBCL) that show a CD20 immunohistochemistry (IHC)-positive and flow cytometry (FC...

BACKGROUND Epidermal Growth Factor Receptor (EGFR) targeting therapies are currently of great relevance for the treatment of lung cancer. For this reason, in addition to mutational analysis immunohistochemistry (IHC) of EGFR in lung cancer has been discussed for the decision making of according therapeutic strategies. The aim of this study was to obtain standardization of EGFR-expression method...

an outbreak of a lethal disease was reported in 4–6-month-old holstein calves in a feedlot around tehran. the signs of central nervous system and gastrointestinal system (gi) involvement were observed in the diseased animals. necropsy samples of gi, liver, kidney, spleen and lung from 3 died animals were prepared for histopathological examination. blood and formalin-fixed ear notch samples of 6...

For several decades, immunohistochemistry (IHC), more specifically diagnostic IHC (dIHC), has been considered an art rather than a laboratory test. There was no clarity about what test performance characteristics are relevant to dIHC, test performance characteristics were not fully defined for dIHC and partly as a consequence of that, there were no standardised controls or reference standards. ...

UNLABELLED Immunohistochemistry (IHC) has been employed in the differential diagnosis of tumors. OBJECTIVE To assess the use of IHC in cases of head and neck tumor. METHOD This is a retrospective study of the cases included in the Cancer Registry of the institution. RESULTS IHC was used in 76 (11%) of 704 pathology tests. Most cases were carcinomas (85.80%), and 83.66% of them were squamo...