Evaluation of B-lymphocytes is one of the most well-established clinical applications of flow cytometric immunophenotyping. This article addresses general principles of the flow cytometric evaluation of B-cell lymphoid neoplasms, followed by discussion of how flow cytometric data can assist in determining a list of diagnostic possibilities and directing additional testing.

Diffuse large B-cell lymphoma in rare instances can present initially in a leukemic phase and mimic other lymphoid diseases. In such cases, advanced diagnostic testing including immunophenotyping, FISH analysis, and karyotyping can help determine the accurate diagnosis which is key in the management of the disease.

Introduction: In 2016, WHO gave a comprehensive classification system that included morphology, immunophenotyping, molecular and genetic findings. Flow cytometric immunophenotyping is the backbone of classification, helping to make more accurate faster diagnosis. Aim: This study has been conducted find aberrancy acute leukemia (AL) its association with gender age group. Material methods: descri...

Background: Leukemias are abnormal proliferation of hematopoietic cells, causing progressive infiltration the marrow. It is eleventh most common cancer in world, and increasingly found now developing countries. Two widely used classifications now, FAB, WHO classification, which has got supplanted with increasing knowledge on cytomorphology cytogenetics. This study, attempts to evaluate role cyt...

Acute myeloblastic leukemia accounts for approximately 20% of acute leukemias in children. The days the microscope represented the main tool in the diagnosis and classification of Acute Myeloblastic Leukemia seem to be very far. This review summarizes the current diagnosis of this malignancy, where the morphological, cytochemical, immunophenotyping, cytogenetic and molecular characterization re...

Laparoscopic surgery plays today an important role in the diagnosis and staging of abdominal lymphomas; in fact it provides adequate lymph node sampling for histological typing and immunophenotyping. The mini-invasive procedure is safe and effective. Intra-operative ultrasound permits to study the parenchimal organs in addition to intra-abdominal lymph node and/or masses.

To evaluate the fully automated FACSCanto software, we compared lymphocyte subpopulation counts obtained using three-color FACSCalibur-CELLQuest and six-color FACSCanto-FACSCanto software techniques. High correlations were observed between data obtained with these techniques. Our study indicated that FACSCanto clinical software is accurate and sensitive in single-platform lymphocyte immunopheno...

Bullet Points The 2008 WHO Classification of Acute Leukemia requires the use of clinical information, morphology, immunophenotyping, cytogenetics and molecular genetics for proper diagnosis and classification Morphology remains essential as the starting point for diagnosis and for directing the ordering of appropriate ancillary studies Proper diagnosis requires evaluation of all data and may re...

Methods A hospital-based case-control study in the northeast region of Brazil. The population included 60 patients diagnosed with non-T acute lymphoid leukemia by means of bone marrow myelogram and immunophenotyping and 120 controls selected proportionally for age and gender. Data collection employed a structured questionnaire to determine prior history of allergies, as well as total IgE serum ...