introduction: serum anti endothelial cell antibodies (aecas) play a prominent role in idiopathic sensorineural hearing loss (snhl) in that they induce vascular damage (immune mediated).the of the current study is  to compare aecas in serum and perilymphatic .uid of idiopathic snhl children (<15y) undergoing cochlear implant surgery.methods: this was a cross sectional study performed in the coch...

Indirect immune fluorescence was used to detect rickettsiae in the tissues of a primate inoculated subcutaneously with Rickettsia rickettsii. Rickettsiae were identified by indirect immuno fluorescence predominantly in skin, skeletal muscle, scrotum, testicles, nares, heart, kidney, liver, brain, spleen, pancreas, and larynx. Cell culture assay confirmed the presence of infectious organisms in ...

In this paper we address the issue of classifying the fluorescent intensity of a sample in Indirect Immuno-Fluorescence (IIF). Since IIF is a subjective, semi-quantitative test in its very nature, we discuss a strategy to reliably label the image data set by using the diagnoses performed by different physicians. Then, we discuss image pre-processing, feature extraction and selection. Finally, w...

In this paper we address the issue of classifying the fluorescent intensity of a sample in Indirect Immuno-Fluorescence (IIF). Since IIF is a subjective, semi-quantitative test in its very nature, we discuss a strategy to reliably label the image data set by using the diagnoses performed by different physicians. Then, we discuss image pre-processing, feature extraction and selection. Finally, w...

Objective Using compound W (a 3,3'-diiodothyronine sulfate [T2S] immuno-crossreactive material)-specific polyclonal antibodies and homogeneous time-resolved fluorescence immunoassay assay techniques (AlphaLISA) to establish an indirect competitive compound W (ICW) quantitative detection method. Method Photosensitive particles (donor beads) coated with compound W or T2S and rabbit anti-W antib...

A highly sensitive and rapid method for the determination of substance P (SP) and streptolysin O (SLO) in human serum is described. The assay is based on enriching the analyte by agglutination/precipitation of immuno-liposomes and enhancing the fluorescence intensity by gramicidin A channels. A mixture of the immuno-liposomes encapsulating a pH-sensitive fluorescence dye BCECF, gramicidin A and...

Bacterial surface proteins are involved in direct contact with host cells and in uptake of nutrients from the environment (1). For this reason, cellular localization can provide insights into the functional role of bacterial proteins. Surface localization of bacterial proteins is a key step towards identification of virulence factors involved in mechanisms of pathogenicity. Methods for fraction...

We have developed an immuno-polymerase chain reaction protocol which includes a highly purified streptavidin-DNA conjugate. The protocol comprises standard ELISA methodology and washing buffers together with a real-time PCR read-out system. The conjugate was employed in both indirect and capture assay formats, which can be completed in a single day using standard laboratory equipment. The immun...