Studies show that Oryza sativa L. contains a structure named β-glucan with anti cancer properties. In the present study, a tissue culture for rice was carried out and β-glucan content was measured under various sugar and vitamin levels in basic MS medium. Seeds of Hashemi cultivar were cultured on MS medium. Two plant hormones, namely, 2,4-D (0, 1, 1.5, 2 and 3 mg/l) and kinetin (0,0.5, 1, and ...

Phagocytes engulf pathogenic microbes, kill them and degrade their cellular macromolecules by hydrolytic enzymes in phagolysosomes. However, such enzymes are unable to degrade some microbial polysaccharides, and fate of such indigestible polysaccharides in phagocytes remains uncertain. Using the extracellular domain of Dectin-1 as β-glucan-specific probes, we succeeded in detection of soluble a...

Background The Bacillus subtilis endo-β-1,4-glucanase (BsCel5A) hydrolyzes β-1,3-1,4-linked glucan, and the enzyme includes a family 3 carbohydrate-binding module (CBM3) that binds β-1,4-linked glucan. Methods Here we investigate the BsCel5A β-1,3-1,4 glucanase activity after exchanging the CBM3 domain for the family 11 CBM from Ruminiclostridium thermocellum celH (RtCBM11) having β-1,3-1,4 g...

Water-insoluble (particulate) β-glucan was isolated from the cell walls of spent brewer’s yeast using a single-step alkaline treatment. To stabilize β-glucan water suspensions, sonication was successfully applied. Th ree diff erent drying methods were used: air-drying, lyophilization and spray-drying. Air-drying and lyophilization caused β-glucan particles agglomeration and changes of their mic...

Hozová B., Kuniak Ľ., Moravčíková P., Gajdošová A. (2007): Determination of water-insoluble β-d-glucan in the whole-grain cereals and pseudocereals. Czech J. Food Sci., 25: 316–324. Water-insoluble β-(1,3)-d-glucan (lichenan) was determined in 43 samples of various cereal (oats, barley, wheat, millet) and pseudocereal (buckwheat, amaranth) cultivars using a modified procedure with fungal α-amyl...

Water-insoluble glucan was isolated from the baker's yeast Saccharomyces cerevisiae. The yeast cells were treated with alkali and the residue then with acid. Chemical and NMR (1D and 2D) analyses showed that a linear (1→3)-β-glucan was purified that was not contaminated with other carbohydrates, proteins or phenolic compounds. The effects of the glucan on wound healing were assessed in human ve...

The anti-inflammatory effect on contact dermatitis of the water solubilized 1'-Acetoxychavicol Acetate (ACA) by complexation with β-1,3-glucan isolated form Aureobasidium pullulans black yeast is reported. It is well-known that ACA possesses a function to inhibit the activation of NF-κB by which genes encoding proinflammatory cytokines, chemokines, and growth factors are regulated. However, bec...

UNLABELLED The walls of infectious pathogens, which are essential for transmission, pathogenesis, and diagnosis, contain sugar polymers that are defining structural features, e.g., β-1,3-glucan and chitin in fungi, chitin in Entamoeba cysts, β-1,3-GalNAc in Giardia cysts, and peptidoglycans in bacteria. The goal here was to determine in which of three walled forms of Toxoplasma gondii (oocyst, ...

The research included 19 breeding lines and 4 cultivars of spring barley from the preliminary field experiments harvested in 2020 Radzików. All samples were characterized for content protein, non-starch polysaccharides (NSP) with soluble (S-NSP) insoluble (I-NSP) fractions β-glucan. Additionally, viscosity water extracts (WEV) was measured to determine functional properties grain. It most diver...

Abstract The basic step in beer production is mashing, during which insoluble starch chains, and to a lesser extent cell walls proteins are broken down by enzymatic hydrolysis. Since the beginning of modern brewing process there have been empirical studies into optimum effective temperatures corresponding enzymes, mashing has carried out accordingly. resulting resting proteolysis, cytolysis amy...