theplant pathogenic phytophthora drechsleri is morphologically similar to some other non-papillate phytophthora spp., especially p. melonis, and it is difficult to discriminate these convergent taxa. it seems that the iranian putative p. drechsleri isolates from different cucurbit species have been generally misidentified and their characteristics do not match with p. drechsleri. in order to co...

abstract- desert truffles are hypogeous ascomyceteous ectomycorrhizal fungi, occurring in arid and semi-arid ecosystems. a pcr-based method was developed for the identification of 3 major desert truffles of iran: terfezia claveryi, tirmania pinoyi and tirmania nivea based on internal transcribed spacers of rdna. two specific pcr primers were designed for t. claveryi, 4 for t. pinoyi, and 2 for ...

objective(s) in addition to several molecular methods and in particular 16s rdna analysis, the application of a more discriminatory genetic marker, i.e., 16s-23s internal transcribed spacer gene sequence has had a great impact on identification and classification of mycobacteria. in the current study we aimed to apply this sequencing power to conclusive identification of some iranian clinical s...

Objective(s) In addition to several molecular methods and in particular 16S rDNA analysis, the application of a more discriminatory genetic marker, i.e., 16S-23S internal transcribed spacer gene sequence has had a great impact on identification and classification of mycobacteria. In the current study we aimed to apply this sequencing power to conclusive identification of some Iranian clinical ...

dunaliella is a green halotolerant microalga, which has several industrial applications e.g. β-carotene production. identification of different dunaliella species has been carried out by morpho-physiological and recently molecular studies. to achieve an improved understanding of taxonomy, these studies are required to be in linkage. the present study describes molecular and specific morpho-phys...

DNA barcode is a very short, standardized DNA sequence in a well-known gene. It provides a way to identify the species to which a plant, animal or fungus it belongs. One of the most popular sequences identification is achieved by comparing DNA sequences of the internal transcribed spacer (ITS) region of nuclear ribosomal cistron. DNA barcoding is a new concept of identifying unknown organisms b...

a specimen of an ascomycetous fungus collected during a field trip was identified as gymnoascus baran. soil samples collected from 0-20 cm depth were studied for isolation of fungi using a soil dilution plate method. based on the morphological and molecular characters the specimens were identified as gymnoascus reesii. genomic dna was extracted and a nuclear rdna region, containing the internal...

background: leishmaniasis is endemic in iran. different species of leishmania (l.) parasites are causative agents of this disease. correct identification of leishmania species is important for clinical studies, prevention, and control of the diseases. mix up of leishmania isolates is possible in the laboratory, so there is need for verification of species for isolates of uncertain identity. dif...

The alignment of the 28S gene of several species of Pezizales allowed to select two pairs of primers able to amplify the internal transcribed spacer region of ribosomal DNA in mycorrhizal fungi, such as truffles. The higher yield of the amplification product demonstrates a better annealing of the new primers to the rDNA, as compared to the universal primers internal transcribed spacer 1 and int...