When two immiscible polymers are brought into intimate contact, highly localized mixing of polymer chains creates an “interphase” region. We have fabricated materials that are entirely interphase by forced assembly of two immiscible polymers. Assemblies of thousands of alternating layers of two polymers, with individual layer thickness on the nanometer size scale of the interphase, were created...

abstract background: to produce a reliable probe suitable for aneuploidy detection of chromosome 13 on uncultured lymphocytes and amniocytes by fluorescence in situ hybridization (fish), we used a contig of three overlapping cosmids mapped to 13q12.3. methods: the cosmid dna carrying the expected sequences of human chromosome 13 was isolated from host cells and labelled with biotin-11-dutp. the...

This study investigates the effectiveness of the antinociceptive effects of diclofenac, an NSAID, on the nociceptive behavior of morphine-treated rats on formalin test.Rats were treated with morphine-containing drinking water for twenty one days, which induced morphine dependence. The antinociceptive effects of 8, 16, and 32 mg/kg doses of diclofenac were then evaluated and compared with distil...

The incorporation of methionine-methyl-(14)C into 18S ribosomal RNA of cultured Chinese hamster ovary cells in early and late interphase has been determined by zone-sedimentation analysis of phenol-extracted RNA preparations. Synchronized cell cultures were prepared for these studies by thymidine treatment and by mechanical selection of mitotic cells. The specific activity of 18S RNA labeled in...

We have quantitatively studied the space-time dynamics of mitotic chromosome compaction in cultured amphibian cells. After collecting digital phase-contrast images we have done digital image analysis to study spatial correlations in density. We find a characteristic distance at which the strongest correlations occur, which provides a quantitative measure of the size of patches of dense chromati...

During interphase, chromosomes are relatively de-condensed in the nuclear space. Interphase chromosomes are known to occupy nuclear space in a non-random manner (chromosome territory); however, their internal structures are poorly defined. In particular, little is understood about the molecular mechanisms that govern the internal organization of interphase chromosomes. The author recently propo...

conventional cytogenetic is the standard technique for detection of philadelphia (ph) chromosome in chronic myeloid leukemia (cml). evaluation of abelson murine leukemia/breakpoint cluster region (abl/bcr) fusion using dual-colour fluorescence in situ hybridization (d-fish) is an alternative approach allowing rapid and reliable detection of the disease. we employed the technique of interphase d...

abstract  background: mosaic form of turner syndrome that represented by two or more  cell lines in an affected individual, often has limitation for detection with classical  cytogenetic methods. the present study was carried out to compare the efficiency of  interphase fluorescence in situ hybridisation (fish) and cytogenetic techniques in  detection of mosaic form of turner syndrome.  methods...

The DNA structure checkpoint protein Rad26ATRIP is also required for an interphase microtubule damage response. This checkpoint delays spindle pole body separation and entry into mitosis following treatment of cells with microtubule poisons. This checkpoint requires cytoplasmic Rad26ATRIP, which is compromised by the rad26:4A allele that inhibits cytoplasmic accum...