While Agrobacterium-mediated transformation (AMT) remains the most widely used technique for gene transfer in plants, interest exists for the use of non-Agrobacterium gene delivery systems due to freedom-to-operate issues that remain with AMT across several jurisdictions. In addition, the plant pathogenic mode of action of Agrobacterium tumefaciens significantly increases the costs to passage e...

BACKGROUND Rice (Oryza sativa L.) is a highly drought sensitive crop, and most semi dwarf rice varieties suffer severe yield losses from reproductive stage drought stress. The genetic complexity of drought tolerance has deterred the identification of agronomically relevant quantitative trait loci (QTL) that can be deployed to improve rice yield under drought in rice. Convergent evidence from ph...

Extreme weather and global warming are drastic agricultural issues causing water scarcity. Under these conditions, it is vital to select drought-tolerant hybrid super rice breeds enhance the yield in dry areas. In current study, a dry-shed experiment was carried out 2019–2020 analyze root growth, gene expression, enzyme activity of Deyou4727, its parent (Dexiang074B * Chenghui727), taking IR64 ...

An indica pyramiding line, DK151, and its recurrent parent, IR64, were evaluated under drought stress and non-stress conditions for three consecutive seasons. DK151 showed significantly improved tolerance to drought. The DNA methylation changes in DK151 and IR64 under drought stress and subsequent recovery were assessed using methylation-sensitive amplified polymorphism analysis. Our results in...

Total spikelet number per panicle (TSN) is one of the most important traits associated with rice yield potential. This trait was assessed in a set of 334 chromosomal segment introgression lines (ILs: BC(3)-derived lines), developed from new plant type (NPT) varieties as donor parents and having the genetic background of an indica-type rice variety IR64. Among the 334 ILs, five lines which had d...

Rice is still play important role as staple food for most of Indonesian population; consequently Indonesia greatly relying on rice production to maintain security. This plant commonly cultivated with intensive use synthetic agrochemicals such chemical fertilizers and pesticides. However, the improper may result in adverse effects environment including biotic abiotic components. study was conduc...

To ensure food security in the face of population growth, decreasing water and land for agriculture, and increasing climate variability, crop yields must increase faster than the current rates. Increased yields will require implementing novel approaches in genetic discovery and breeding. Here we demonstrate the potential of field-based high throughput phenotyping (HTP) on a large recombinant po...

Differences in drought stress tolerance within diverse rice genotypes have been attributed to genetic diversity and epigenetic alterations. DNA methylation is an important epigenetic modification that influences diverse biological processes, but its effects on rice drought stress tolerance are poorly understood. In this study, methylated DNA immunoprecipitation sequencing and an Affymetrix Gene...

Abstract Aims Growth reductions and yield losses from drought could be mitigated by developing rice genotypes with more efficient root systems. We examined spatiotemporal responses to in order determine whether roots upper vs. deeper soil layers respond differently stress. Methods Root anatomical architectural phenotypes of two genotypes, Azucena (drought tolerant) IR64 susceptible), were measu...

In future climates, greater heat tolerance at anthesis will be required in rice. The effect of high temperature at anthesis on spikelet fertility was studied on IR64 (lowland indica) and Azucena (upland japonica) at 29.6 degrees C (control), 33.7 degrees C, and 36.2 degrees C tissue temperatures. The objectives of the study were to: (i) determine the effect of temperature on flowering pattern; ...