نتایج جستجو برای: is50 pcr

تعداد نتایج: 175108  

Journal: :Genome Research 1994

Journal: :PCR methods and applications 1994
D E Lanar K C Kain

1Department of Immunology, Waiter Reed Army Institute of Research, Washington, D.C. 20307-5100; 2Tropical Disease Unit, Division of Infectious Diseases, The Toronto Hospital and The University of Toronto, Toronto, Ontario, Canada M5G 2C4 In vitro transcription and translation are powerful tools for examining the s t ructure-funct ion relationships of proteins. Genes can be cloned into plasmid v...

2004
Natalia E. Broude Sjaak van Heusden Richard Finkers

We present a protocol for effi cient amplifi cation of a large number of DNA targets using a single-tube polymerase chain reaction (PCR) based on PCR suppression. This method allows amplifi cation of each DNA target with only one target-specifi c primer whereas the other primer is common for all targets. Thus, this approach requires n+1 primers for n targets instead of 2n in conventional PCR an...

Journal: :PCR methods and applications 1993
M Clementi S Menzo P Bagnarelli A Manzin A Valenza P E Varaldo

I n the last few years, molecular hybridization methods have been used extensively in basic and applied virology because of their technical flexibility and high specificity. Using these techniques, the detection of DNA and RNA viruses directly from clinical specimens, the analysis of the specific transcriptional activity of viral genes in vitro and in vivo, and the study of virus-host relations...

Journal: :The Journal of neuroscience : the official journal of the Society for Neuroscience 2013
Davi J A Moraes Melina P da Silva Leni G H Bonagamba André S Mecawi Daniel B Zoccal José Antunes-Rodrigues Wamberto A Varanda Benedito H Machado

The respiratory pattern generator modulates the sympathetic outflow, the strength of which is enhanced by challenges produced by hypoxia. This coupling is due to the respiratory-modulated presympathetic neurons in the rostral ventrolateral medulla (RVLM), but the underlining electrophysiological mechanisms remain unclear. For a better understanding of the neural substrates responsible for gener...

Journal: :Bioinformatics 2010
Eduardo Gorrón Fausto Rodríguez Diana Bernal Luis Miguel Rodriguez-Rojas Adriana Bernal Silvia Restrepo Joe Tohme

MOTIVATION We developed a technique and a tool for degenerate primer design based on multiple local alignments employing the MEME algorithm supported with electronic PCR. The objective is to find adequate primers starting from sequences with poor global similarity. We show an example of its application in our laboratory to find sequences in Brachiaria with similarity to ESTs related to apomixis.

Journal: :Acta poloniae pharmaceutica 2008
Sławomir Smolik Dorota Domal-Kwiatkowska Ewa Nowalany-Kozielska Romuald Wojnicz Longina Swiatowska Weglarz Ludmiła

Inflammatory cytokines have an important role in the pathogenesis of myocarditis, but still little is known about the importance of interferon gamma (IFNg) in this disease. The aim of the study was to evaluate the prognostic value of the initial transcriptional activity of IFNg and two subunits of its receptor as measured with the use of QRT-PCR and SYBRGreen chemistry in the group of 63 patien...

Journal: :Nucleic acids research 1997
H Pues B Holz E Weinhold

A quick in vitro mutagenesis method for the construction of nested deletion libraries was developed. Many deletions can be obtained in a single inverse PCR (IPCR) by replacing one of the two primers with a mixture of 5'-truncated oligodeoxynucleotides. Since chemical DNA synthesis proceeds from the 3'to the 5'end, such a mixture of 5'-truncated oligodeoxynucleotides can easily be obtained in a ...

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